Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates
Monoclonal antibodies (MAbs) specific to plum pox virus (PPV) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (CP). The characterized MAbs could be used i...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5535 |
| _version_ | 1855491941705187328 |
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| author | López-Moya, Juan J. Sanz, Antonio Cambra, Mariano Gorris, María T. Anaya, C. Miguet, J. G. Cortes, E. Lopez Abella, D. |
| author_browse | Anaya, C. Cambra, Mariano Cortes, E. Gorris, María T. Lopez Abella, D. López-Moya, Juan J. Miguet, J. G. Sanz, Antonio |
| author_facet | López-Moya, Juan J. Sanz, Antonio Cambra, Mariano Gorris, María T. Anaya, C. Miguet, J. G. Cortes, E. Lopez Abella, D. |
| author_sort | López-Moya, Juan J. |
| collection | ReDivia |
| description | Monoclonal antibodies (MAbs) specific to plum pox virus (PPV) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (CP). The characterized MAbs could be used in ELISA to differentiate several Mediterranean PPV isolates differing in their geographical origin and CP size. At least seven antigenic sites could be established based on the recognition pattern and competition binding analysis, and the epitopes could be classified in three groups by Western blot analysis of intact and trypsin digested virus particles. By means of electron microscopy the epitopes could be seen to be located on the surface of the virus particles. |
| format | Artículo |
| id | ReDivia5535 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55352025-04-25T14:43:13Z Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates López-Moya, Juan J. Sanz, Antonio Cambra, Mariano Gorris, María T. Anaya, C. Miguet, J. G. Cortes, E. Lopez Abella, D. Monoclonal antibodies (MAbs) specific to plum pox virus (PPV) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (CP). The characterized MAbs could be used in ELISA to differentiate several Mediterranean PPV isolates differing in their geographical origin and CP size. At least seven antigenic sites could be established based on the recognition pattern and competition binding analysis, and the epitopes could be classified in three groups by Western blot analysis of intact and trypsin digested virus particles. By means of electron microscopy the epitopes could be seen to be located on the surface of the virus particles. 2017-06-01T10:12:31Z 2017-06-01T10:12:31Z 1994 1994 article Lopezmoya, J.J., Sanz, A., Cambra, M., Gorris, M. T., Anaya, C., Miguet, J. G., Cortes, E., Lopezabella, D. (1994). Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates. Archives of Virology, 135(3-4), 293-304. 0304-8608 http://hdl.handle.net/20.500.11939/5535 10.1007/BF01310015 en openAccess Impreso |
| spellingShingle | López-Moya, Juan J. Sanz, Antonio Cambra, Mariano Gorris, María T. Anaya, C. Miguet, J. G. Cortes, E. Lopez Abella, D. Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title | Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title_full | Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title_fullStr | Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title_full_unstemmed | Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title_short | Production and Characterization of Monoclonal-Antibodies to Plum Pox Virus and their use in Differentiation of Mediterranean Isolates |
| title_sort | production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates |
| url | http://hdl.handle.net/20.500.11939/5535 |
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