Production of tetraploid plants of non apomictic citrus genotypes
Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid va...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5532 |
| _version_ | 1855491941152587776 |
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| author | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_browse | Aleza, Pablo Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x-4x). These stable tetraploid plants have been used in 4x x 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years. |
| format | Artículo |
| id | ReDivia5532 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55322025-04-25T14:43:13Z Production of tetraploid plants of non apomictic citrus genotypes Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization of mixoploids plants (2x-4x). These stable tetraploid plants have been used in 4x x 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years. 2017-06-01T10:12:31Z 2017-06-01T10:12:31Z 2009 DEC article Aleza, P., Juárez, J., Ollitrault, P., & Navarro, L. (2009). Production of tetraploid plants of non apomictic citrus genotypes. Plant cell reports, 28(12), 1837-1846. 0721-7714 http://hdl.handle.net/20.500.11939/5532 10.1007/s00299-009-0783-2 en openAccess Impreso |
| spellingShingle | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Production of tetraploid plants of non apomictic citrus genotypes |
| title | Production of tetraploid plants of non apomictic citrus genotypes |
| title_full | Production of tetraploid plants of non apomictic citrus genotypes |
| title_fullStr | Production of tetraploid plants of non apomictic citrus genotypes |
| title_full_unstemmed | Production of tetraploid plants of non apomictic citrus genotypes |
| title_short | Production of tetraploid plants of non apomictic citrus genotypes |
| title_sort | production of tetraploid plants of non apomictic citrus genotypes |
| url | http://hdl.handle.net/20.500.11939/5532 |
| work_keys_str_mv | AT alezapablo productionoftetraploidplantsofnonapomicticcitrusgenotypes AT juarezjose productionoftetraploidplantsofnonapomicticcitrusgenotypes AT ollitraultpatrick productionoftetraploidplantsofnonapomicticcitrusgenotypes AT navarroluis productionoftetraploidplantsofnonapomicticcitrusgenotypes |