Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication.
The Arabis mosaic virus (ArMV) is one of the causative agent of the grapevine fanleaf disease, one of the most widespread and damaging viral diseases of grapevine. Recently, the ArMV has been detected in Spanish vineyards, and its determination and molecular characterization was undertaken. To this...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
|
| Online Access: | http://hdl.handle.net/20.500.11939/5526 |
| _version_ | 1855491940214112256 |
|---|---|
| author | López-Fabuel, Irene Olmos, Antonio Bassler, Alexandra Dupuis-Maguiraga, L. Torres, Luis B. Bertolini, Edson Wetzel, Thierry |
| author_browse | Bassler, Alexandra Bertolini, Edson Dupuis-Maguiraga, L. López-Fabuel, Irene Olmos, Antonio Torres, Luis B. Wetzel, Thierry |
| author_facet | López-Fabuel, Irene Olmos, Antonio Bassler, Alexandra Dupuis-Maguiraga, L. Torres, Luis B. Bertolini, Edson Wetzel, Thierry |
| author_sort | López-Fabuel, Irene |
| collection | ReDivia |
| description | The Arabis mosaic virus (ArMV) is one of the causative agent of the grapevine fanleaf disease, one of the most widespread and damaging viral diseases of grapevine. Recently, the ArMV has been detected in Spanish vineyards, and its determination and molecular characterization was undertaken. To this aim, the nucleotide sequence of the genomic RNAs 1 and 2 of the first isolate of ArMV infecting grapevine detected in Spain (ArMV-DU13) has been determined. The ArMV-DU13 genomic sequences were compared to the corresponding sequences of other isolates of ArMV, or nepoviruses. The most divergent genes among ArMV isolates were the X1 and VPg genes on the RNA 1, and the 2A gene on the RNA 2, with identity levels at the amino acid level of 78% (X1 and VPg) or 69% (2A) between the most distant isolates. Interestingly, the VPg genes were identical between the two grapevine isolates ArMV-Du13 and -NW, suggesting a possible implication of the host. The phylogenetic analysis of the RNA 2 showed that the Spanish isolate was close to Grapevine fanleaf virus isolates. The analysis of the full length RNA 2 suggests a recombination event between ArMV-DU13 and GFLV-GHu isolates between nucleotides 54 and 586 in the ArMV-DU13 isolate. Altogether, these results confirm the high variability between isolates of ArMV, and will be helpful to design more appropriate and reliable molecular diagnostic techniques for the control of this emerging virus in Spain. |
| format | Artículo |
| id | ReDivia5526 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55262025-04-25T14:43:12Z Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. López-Fabuel, Irene Olmos, Antonio Bassler, Alexandra Dupuis-Maguiraga, L. Torres, Luis B. Bertolini, Edson Wetzel, Thierry The Arabis mosaic virus (ArMV) is one of the causative agent of the grapevine fanleaf disease, one of the most widespread and damaging viral diseases of grapevine. Recently, the ArMV has been detected in Spanish vineyards, and its determination and molecular characterization was undertaken. To this aim, the nucleotide sequence of the genomic RNAs 1 and 2 of the first isolate of ArMV infecting grapevine detected in Spain (ArMV-DU13) has been determined. The ArMV-DU13 genomic sequences were compared to the corresponding sequences of other isolates of ArMV, or nepoviruses. The most divergent genes among ArMV isolates were the X1 and VPg genes on the RNA 1, and the 2A gene on the RNA 2, with identity levels at the amino acid level of 78% (X1 and VPg) or 69% (2A) between the most distant isolates. Interestingly, the VPg genes were identical between the two grapevine isolates ArMV-Du13 and -NW, suggesting a possible implication of the host. The phylogenetic analysis of the RNA 2 showed that the Spanish isolate was close to Grapevine fanleaf virus isolates. The analysis of the full length RNA 2 suggests a recombination event between ArMV-DU13 and GFLV-GHu isolates between nucleotides 54 and 586 in the ArMV-DU13 isolate. Altogether, these results confirm the high variability between isolates of ArMV, and will be helpful to design more appropriate and reliable molecular diagnostic techniques for the control of this emerging virus in Spain. 2017-06-01T10:12:31Z 2017-06-01T10:12:31Z 2013 MAR 2013 article publishedVersion Lopez-Fabuel, I., Olmos, A., Bassler, A., Dupuis-Maguiraga, L., Torres, L. B., Bertolini, E. et al. (2013). Short communication. Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Spanish Journal of Agricultural Research, 11(1), 199-203. 1695-971X http://hdl.handle.net/20.500.11939/5526 10.5424/sjar/2013111-3069 en openAccess Impreso |
| spellingShingle | López-Fabuel, Irene Olmos, Antonio Bassler, Alexandra Dupuis-Maguiraga, L. Torres, Luis B. Bertolini, Edson Wetzel, Thierry Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title | Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title_full | Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title_fullStr | Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title_full_unstemmed | Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title_short | Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines. Short communication. |
| title_sort | molecular analysis of the genomic rnas 1 and 2 of the first arabis mosaic virus isolate detected in spanish grapevines short communication |
| url | http://hdl.handle.net/20.500.11939/5526 |
| work_keys_str_mv | AT lopezfabuelirene molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT olmosantonio molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT bassleralexandra molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT dupuismaguiragal molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT torresluisb molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT bertoliniedson molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication AT wetzelthierry molecularanalysisofthegenomicrnas1and2ofthefirstarabismosaicvirusisolatedetectedinspanishgrapevinesshortcommunication |