Polyembryonyin Non-apomictic Citrus Genotypes
Adventitious embryony from nucellar cells is the mechanism leading to apomixis in citrus. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x×4x hybridisations and in vitro culture of isolated nucellus. The origin of the plants obtained a...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5521 |
| _version_ | 1855032292083236864 |
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| author | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_browse | Aleza, Pablo Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Adventitious embryony from nucellar cells is the mechanism
leading to apomixis in citrus. However, singular cases
of polyembryony have been reported in non-apomictic
genotypes as a consequence of 2x×4x hybridisations and in
vitro culture of isolated nucellus. The origin of the plants
obtained as a consequence of these two processes is still
unclear. In this work, we systematically analyzed the
genetic structure (ploidy and allelic constitution at SSR
locus) of plants obtained from polyembryonic seeds arising
from 2x×4x sexual hybridisations or regenerated from
nucellus culture in vitro of different non-apomictic citrus
genotypes. Histological studies were also conduced to try to
identify the initiation process of polyembryony in nonapomictic
genotypes. We demonstrate that all plants
obtained from the same undeveloped seed in 2x×4x
hybridisations resulted from fission of the original zygotic
embryo. Also, the plants obtained from in vitro culture of
nucellus were recovered by somatic embryogenesis from
cells having the same genotype as the zygotic embryos of
the same seed. It appears that in non-apomictic citrus,
proembryos or embryogenic cells are formed by fission of
the original zygotic embryo and that the development of
these adventitious embryos, normally hampered, can take
place in vivo or in vitro as result of two different mechanisms that prevent the dominance of the initial
zygotic embryo. |
| format | article |
| id | ReDivia5521 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55212025-04-25T14:43:11Z Polyembryonyin Non-apomictic Citrus Genotypes Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Adventitious embryony from nucellar cells is the mechanism leading to apomixis in citrus. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x×4x hybridisations and in vitro culture of isolated nucellus. The origin of the plants obtained as a consequence of these two processes is still unclear. In this work, we systematically analyzed the genetic structure (ploidy and allelic constitution at SSR locus) of plants obtained from polyembryonic seeds arising from 2x×4x sexual hybridisations or regenerated from nucellus culture in vitro of different non-apomictic citrus genotypes. Histological studies were also conduced to try to identify the initiation process of polyembryony in nonapomictic genotypes. We demonstrate that all plants obtained from the same undeveloped seed in 2x×4x hybridisations resulted from fission of the original zygotic embryo. Also, the plants obtained from in vitro culture of nucellus were recovered by somatic embryogenesis from cells having the same genotype as the zygotic embryos of the same seed. It appears that in non-apomictic citrus, proembryos or embryogenic cells are formed by fission of the original zygotic embryo and that the development of these adventitious embryos, normally hampered, can take place in vivo or in vitro as result of two different mechanisms that prevent the dominance of the initial zygotic embryo. 2017-06-01T10:12:30Z 2017-06-01T10:12:30Z 2010 SPR article Aleza, P., Juarez, J., Ollitrault, P., & Navarro, L. (2010). Polyembryonyin non-apomictic citrus genotypes. In Vitro Cellular & Developmental Biology-Animal, 46, S145-S145. 1071-2690 http://hdl.handle.net/20.500.11939/5521 10.1007/s11626-010-9339-6 en openAccess Impreso |
| spellingShingle | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Polyembryonyin Non-apomictic Citrus Genotypes |
| title | Polyembryonyin Non-apomictic Citrus Genotypes |
| title_full | Polyembryonyin Non-apomictic Citrus Genotypes |
| title_fullStr | Polyembryonyin Non-apomictic Citrus Genotypes |
| title_full_unstemmed | Polyembryonyin Non-apomictic Citrus Genotypes |
| title_short | Polyembryonyin Non-apomictic Citrus Genotypes |
| title_sort | polyembryonyin non apomictic citrus genotypes |
| url | http://hdl.handle.net/20.500.11939/5521 |
| work_keys_str_mv | AT alezapablo polyembryonyinnonapomicticcitrusgenotypes AT juarezjose polyembryonyinnonapomicticcitrusgenotypes AT ollitraultpatrick polyembryonyinnonapomicticcitrusgenotypes AT navarroluis polyembryonyinnonapomicticcitrusgenotypes |