European protocol for diagnosis of Erwinia amylovora
The EU-funded project DIAGPRO has developed diagnostic protocols for 18 quarantine pests including Erwinia amylovora. The protocol for this organism includes a diagnosis scheme for detection of the bacterium in symptomatic and asymptomatic plants with details of the different techniques as isolation...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Objeto de conferencia |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5519 |
| _version_ | 1855491938895003648 |
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| author | López, María M. Llop, Pablo Gorris, María T. Penyalver, Javier Donat, Victoria Cambra, Mariano Keck, M. |
| author2 | Bazzi, C. Mazzucchi, U. |
| author_browse | Bazzi, C. Mazzucchi, U. Cambra, Mariano Donat, Victoria Gorris, María T. Keck, M. Llop, Pablo López, María M. Penyalver, Javier |
| author_facet | Bazzi, C. Mazzucchi, U. López, María M. Llop, Pablo Gorris, María T. Penyalver, Javier Donat, Victoria Cambra, Mariano Keck, M. |
| author_sort | López, María M. |
| collection | ReDivia |
| description | The EU-funded project DIAGPRO has developed diagnostic protocols for 18 quarantine pests including Erwinia amylovora. The protocol for this organism includes a diagnosis scheme for detection of the bacterium in symptomatic and asymptomatic plants with details of the different techniques as isolation in three media, direct tissue print-ELISA, enrichment DASI-ELISA, immunofluorescence, conventional PCR and nested-PCR in a single closed tube (before and after enrichment of the samples in two liquid media). A ring test was conducted to evaluate and standardize some techniques and protocols. Testing was performed independently at 10 plant health laboratories analysing 15 samples (healthy, spiked or inoculated). The reliability of the diagnosis across all laboratories decreased with inoculum concentration. The accuracy of the different techniques, defined as (true positive diagnosis + true negatives)/total number of samples, was: 0.88, 0.92 and 0.92 respectively for isolation on King’s B, SNA and CCT media; 0.70, 0.72 and 0.66 for immunofluorescence performed with one monoclonal and two polyclonal antibodies commercially available; 0.79 and 0.83 for enrichment-ELISA with a commercial kit after enrichment in two media; 0.51 for conventional PCR and 0.69 for nested-PCR in a single tube, both techniques increasing their accuracy up to 0.78 and 0.86, respectively, after enrichment. The designed protocol is freely available at the web site: www.csl.gov.uk/science/organ/ph/diagpro. |
| format | Objeto de conferencia |
| id | ReDivia5519 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55192025-04-25T14:53:26Z European protocol for diagnosis of Erwinia amylovora ACTA HORTICULTURAE López, María M. Llop, Pablo Gorris, María T. Penyalver, Javier Donat, Victoria Cambra, Mariano Keck, M. Bazzi, C. Mazzucchi, U. The EU-funded project DIAGPRO has developed diagnostic protocols for 18 quarantine pests including Erwinia amylovora. The protocol for this organism includes a diagnosis scheme for detection of the bacterium in symptomatic and asymptomatic plants with details of the different techniques as isolation in three media, direct tissue print-ELISA, enrichment DASI-ELISA, immunofluorescence, conventional PCR and nested-PCR in a single closed tube (before and after enrichment of the samples in two liquid media). A ring test was conducted to evaluate and standardize some techniques and protocols. Testing was performed independently at 10 plant health laboratories analysing 15 samples (healthy, spiked or inoculated). The reliability of the diagnosis across all laboratories decreased with inoculum concentration. The accuracy of the different techniques, defined as (true positive diagnosis + true negatives)/total number of samples, was: 0.88, 0.92 and 0.92 respectively for isolation on King’s B, SNA and CCT media; 0.70, 0.72 and 0.66 for immunofluorescence performed with one monoclonal and two polyclonal antibodies commercially available; 0.79 and 0.83 for enrichment-ELISA with a commercial kit after enrichment in two media; 0.51 for conventional PCR and 0.69 for nested-PCR in a single tube, both techniques increasing their accuracy up to 0.78 and 0.86, respectively, after enrichment. The designed protocol is freely available at the web site: www.csl.gov.uk/science/organ/ph/diagpro. 2017-06-01T10:12:30Z 2017-06-01T10:12:30Z 2006 2006 conferenceObject Lopez, M. M., Llop, P., Gorris, M. T., Penalver, J., Donat, V., Cambra, M. et al. (2006). European protocol for diagnosis of Erwinia amylovora. Proceedings of the Xth International Workshop on Fire Blight, (704), 99-103. 0567-7572; 90-6605-407-7 http://hdl.handle.net/20.500.11939/5519 10.17660/ActaHortic.2006.704.11 en openAccess Impreso |
| spellingShingle | López, María M. Llop, Pablo Gorris, María T. Penyalver, Javier Donat, Victoria Cambra, Mariano Keck, M. European protocol for diagnosis of Erwinia amylovora |
| title | European protocol for diagnosis of Erwinia amylovora |
| title_full | European protocol for diagnosis of Erwinia amylovora |
| title_fullStr | European protocol for diagnosis of Erwinia amylovora |
| title_full_unstemmed | European protocol for diagnosis of Erwinia amylovora |
| title_short | European protocol for diagnosis of Erwinia amylovora |
| title_sort | european protocol for diagnosis of erwinia amylovora |
| url | http://hdl.handle.net/20.500.11939/5519 |
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