Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria
The detection of low numbers of plant pathogenic bacteria can be improved by using a combination of enrichment in selective media and specific monoclonal antibodies (mab) or specific primers. In this way, the enrichment increases the number of the target bacteria and the specific probes can accurate...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5515 |
| _version_ | 1855491938137931776 |
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| author | López, María M. Gorris, María T. Llop, Pablo Cubero, Jaime Vicedo, Begonya Cambra, Mariano |
| author2 | Dehne, HW Adam, G Diekmann, M Frahm, J MaulerMachnik, A VanHalteren, P. |
| author_browse | Cambra, Mariano Cubero, Jaime Dehne, HW Adam, G Diekmann, M Frahm, J MaulerMachnik, A VanHalteren, P. Gorris, María T. Llop, Pablo López, María M. Vicedo, Begonya |
| author_facet | Dehne, HW Adam, G Diekmann, M Frahm, J MaulerMachnik, A VanHalteren, P. López, María M. Gorris, María T. Llop, Pablo Cubero, Jaime Vicedo, Begonya Cambra, Mariano |
| author_sort | López, María M. |
| collection | ReDivia |
| description | The detection of low numbers of plant pathogenic bacteria can be improved by using a combination of enrichment in selective media and specific monoclonal antibodies (mab) or specific primers. In this way, the enrichment increases the number of the target bacteria and the specific probes can accurately identify them in spite of some increase of the total bacterial population in the samples. The enrichment step must be optimised for each bacterium by defining the medium, time, temperature and incubation conditions in order to obtain high numbers of the target bacterium that will easily detected by the specific probes in a second step. Such procedures have been applied to the detection of Agrobacterium tumefaciens causal agent of crown gall, and to the detection of Erwinia carotovora subsp. atroseptica casual agent of potato black leg and E. amylovora casual agent of fire blight. |
| format | Artículo |
| id | ReDivia5515 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55152025-04-25T14:43:10Z Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria DEVELOPMENTS IN PLANT PATHOLOGY López, María M. Gorris, María T. Llop, Pablo Cubero, Jaime Vicedo, Begonya Cambra, Mariano Dehne, HW Adam, G Diekmann, M Frahm, J MaulerMachnik, A VanHalteren, P. The detection of low numbers of plant pathogenic bacteria can be improved by using a combination of enrichment in selective media and specific monoclonal antibodies (mab) or specific primers. In this way, the enrichment increases the number of the target bacteria and the specific probes can accurately identify them in spite of some increase of the total bacterial population in the samples. The enrichment step must be optimised for each bacterium by defining the medium, time, temperature and incubation conditions in order to obtain high numbers of the target bacterium that will easily detected by the specific probes in a second step. Such procedures have been applied to the detection of Agrobacterium tumefaciens causal agent of crown gall, and to the detection of Erwinia carotovora subsp. atroseptica casual agent of potato black leg and E. amylovora casual agent of fire blight. 2017-06-01T10:12:29Z 2017-06-01T10:12:29Z 1997 1997 article Lopez, M.M., Gorris, M. T., Llop, P., Cubero, J., Vicedo, B., Cambra, M. (1997). Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria. Diagnosis and Identification of Plant Pathogens, 11, 117-121. 0929-1318; 0-7923-4771-4 http://hdl.handle.net/20.500.11939/5515 10.1007/978-94-009-0043-1_25 en openAccess Impreso |
| spellingShingle | López, María M. Gorris, María T. Llop, Pablo Cubero, Jaime Vicedo, Begonya Cambra, Mariano Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title | Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title_full | Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title_fullStr | Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title_full_unstemmed | Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title_short | Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria |
| title_sort | selective enrichment improves isolation serological and molecular detection of plant pathogenic bacteria |
| url | http://hdl.handle.net/20.500.11939/5515 |
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