Innovative tools for detection of plant pathogenic viruses and bacteria
Detection of harmful viruses and bacteria in plant material, vectors or natural reservoirs is essential to ensure safe and sustainable agriculture. The techniques available have evolved significantly in the last few years to achieve rapid and reliable detection of pathogens, extraction of the target...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5512 |
| _version_ | 1855032290618376192 |
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| author | López, María M. Bertolini, Edson Olmos, Antonio Caruso, Paola Gorris, María T. Llop, Pablo Penyalver, Ramón Cambra, Mariano |
| author_browse | Bertolini, Edson Cambra, Mariano Caruso, Paola Gorris, María T. Llop, Pablo López, María M. Olmos, Antonio Penyalver, Ramón |
| author_facet | López, María M. Bertolini, Edson Olmos, Antonio Caruso, Paola Gorris, María T. Llop, Pablo Penyalver, Ramón Cambra, Mariano |
| author_sort | López, María M. |
| collection | ReDivia |
| description | Detection of harmful viruses and bacteria in plant material, vectors or natural reservoirs is essential to ensure safe and sustainable agriculture. The techniques available have evolved significantly in the last few years to achieve rapid and reliable detection of pathogens, extraction of the target from the, sample being important for optimising detection. For viruses, sample preparation has been simplified by imprinting or squashing plant material or insect vectors onto membranes. To improve the sensitivity of techniques for bacterial detection, a prior enrichment step in liquid or solid medium is advised. Serological and molecular techniques are currently the most appropriate when high numbers of samples need to be analysed. Specific monoclonal and/or recombinant antibodies are available for many plant pathogens and have contributed to the specificity of serological detection. Molecular detection can be optimised through the automatic purification of nucleic acids from pathogens by columns or robotics. New variants of PCR, such as simple or multiplex nested PCR in a single closed tube, co-operative-PCR and real-time monitoring of amplicons or quantitative PCR, allow high sensitivity in the detection of one or several pathogens in a single assay. The latest development in the analysis of nucleic acids is micro-array technology, but it requires generic DNA/RNA extraction and pre-amplification methods to increase detection sensitivity. The advances in research that will result from the sequencing of many plant pathogen genomes, especially now in the era of proteomics, represent a new source of information for the future development of sensitive and specific detection techniques for these microorganisms. |
| format | article |
| id | ReDivia5512 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55122025-04-25T14:43:10Z Innovative tools for detection of plant pathogenic viruses and bacteria López, María M. Bertolini, Edson Olmos, Antonio Caruso, Paola Gorris, María T. Llop, Pablo Penyalver, Ramón Cambra, Mariano Detection of harmful viruses and bacteria in plant material, vectors or natural reservoirs is essential to ensure safe and sustainable agriculture. The techniques available have evolved significantly in the last few years to achieve rapid and reliable detection of pathogens, extraction of the target from the, sample being important for optimising detection. For viruses, sample preparation has been simplified by imprinting or squashing plant material or insect vectors onto membranes. To improve the sensitivity of techniques for bacterial detection, a prior enrichment step in liquid or solid medium is advised. Serological and molecular techniques are currently the most appropriate when high numbers of samples need to be analysed. Specific monoclonal and/or recombinant antibodies are available for many plant pathogens and have contributed to the specificity of serological detection. Molecular detection can be optimised through the automatic purification of nucleic acids from pathogens by columns or robotics. New variants of PCR, such as simple or multiplex nested PCR in a single closed tube, co-operative-PCR and real-time monitoring of amplicons or quantitative PCR, allow high sensitivity in the detection of one or several pathogens in a single assay. The latest development in the analysis of nucleic acids is micro-array technology, but it requires generic DNA/RNA extraction and pre-amplification methods to increase detection sensitivity. The advances in research that will result from the sequencing of many plant pathogen genomes, especially now in the era of proteomics, represent a new source of information for the future development of sensitive and specific detection techniques for these microorganisms. 2017-06-01T10:12:29Z 2017-06-01T10:12:29Z 2003 DEC 2003 article López, M. M., Bertolini, E., Olmos, A., Caruso, P., Gorris, M. T., Llop, P. et al. (2003). Innovative tools for detection of plant pathogenic viruses and bacteria. International Microbiology, 6(4), 233-243. 1139-6709 http://hdl.handle.net/20.500.11939/5512 10.1007/s10123-003-0143-y en openAccess Impreso |
| spellingShingle | López, María M. Bertolini, Edson Olmos, Antonio Caruso, Paola Gorris, María T. Llop, Pablo Penyalver, Ramón Cambra, Mariano Innovative tools for detection of plant pathogenic viruses and bacteria |
| title | Innovative tools for detection of plant pathogenic viruses and bacteria |
| title_full | Innovative tools for detection of plant pathogenic viruses and bacteria |
| title_fullStr | Innovative tools for detection of plant pathogenic viruses and bacteria |
| title_full_unstemmed | Innovative tools for detection of plant pathogenic viruses and bacteria |
| title_short | Innovative tools for detection of plant pathogenic viruses and bacteria |
| title_sort | innovative tools for detection of plant pathogenic viruses and bacteria |
| url | http://hdl.handle.net/20.500.11939/5512 |
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