Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime
P>Mexican lime plants transformed with the 3'-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations f...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5511 |
| _version_ | 1855491937543389184 |
|---|---|
| author | Lopez, Carmelo Cervera, Magdalena Fagoaga, Carmen Moreno, Pedro Navarro, Luis Flores, Ricardo Pena, Leandro |
| author_browse | Cervera, Magdalena Fagoaga, Carmen Flores, Ricardo Lopez, Carmelo Moreno, Pedro Navarro, Luis Pena, Leandro |
| author_facet | Lopez, Carmelo Cervera, Magdalena Fagoaga, Carmen Moreno, Pedro Navarro, Luis Flores, Ricardo Pena, Leandro |
| author_sort | Lopez, Carmelo |
| collection | ReDivia |
| description | P>Mexican lime plants transformed with the 3'-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%-56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs. |
| format | Artículo |
| id | ReDivia5511 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55112025-04-25T14:43:09Z Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime Lopez, Carmelo Cervera, Magdalena Fagoaga, Carmen Moreno, Pedro Navarro, Luis Flores, Ricardo Pena, Leandro P>Mexican lime plants transformed with the 3'-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%-56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs. 2017-06-01T10:12:29Z 2017-06-01T10:12:29Z 2010 JAN 2010 article publishedVersion Lopez, Carmelo, Cervera, M., Fagoaga, C., Moreno, P., Navarro, L., Flores, R., Pena, L. (2010). Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime. Molecular Plant Pathology, 11(1), 33-41. 1464-6722 http://hdl.handle.net/20.500.11939/5511 10.1111/j.1364-3703.2009.00566.X en openAccess Impreso |
| spellingShingle | Lopez, Carmelo Cervera, Magdalena Fagoaga, Carmen Moreno, Pedro Navarro, Luis Flores, Ricardo Pena, Leandro Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title | Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title_full | Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title_fullStr | Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title_full_unstemmed | Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title_short | Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime |
| title_sort | accumulation of transgene derived sirnas is not sufficient for rnai mediated protection against citrus tristeza virus in transgenic mexican lime |
| url | http://hdl.handle.net/20.500.11939/5511 |
| work_keys_str_mv | AT lopezcarmelo accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT cerveramagdalena accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT fagoagacarmen accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT morenopedro accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT navarroluis accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT floresricardo accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime AT penaleandro accumulationoftransgenederivedsirnasisnotsufficientforrnaimediatedprotectionagainstcitrustristezavirusintransgenicmexicanlime |