Polyembryony in non-apomictic citrus genotypes
Adventitious embryony from nucellar cells is the mechanism leading to apomixis in Citrus sp. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x x 4x hybridizations and in vitro culture of isolated nucelli. The origin of the plants arising fro...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5510 |
| _version_ | 1855491937359888384 |
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| author | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_browse | Aleza, Pablo Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Adventitious embryony from nucellar cells is the mechanism leading to apomixis in Citrus sp. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x x 4x hybridizations and in vitro culture of isolated nucelli. The origin of the plants arising from the aforementioned processes remains unclear. The genetic structure (ploidy and allelic constitution with microsatellite markers) of plants obtained from polyembryonic seeds arising from 2x x 4x sexual hybridizations and those regenerated from nucellus culture in vitro was systematically analysed in different non-apomictic citrus genotypes. Histological studies were also conducted to try to identify the initiation process underlying polyembryony. All plants obtained from the same undeveloped seed in 2x x 4x hybridizations resulted from cleavage of the original zygotic embryo. Also, the plants obtained from in vitro nucellus culture were recovered by somatic embryogenesis from cells that shared the same genotype as the zygotic embryos of the same seed. It appears that in non-apomictic citrus genotypes, proembryos or embryogenic cells are formed by cleavage of the zygotic embryos and that the development of these adventitious embryos, normally hampered, can take place in vivo or in vitro as a result of two different mechanisms that prevent the dominance of the initial zygotic embryo. |
| format | Artículo |
| id | ReDivia5510 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55102025-04-25T14:43:09Z Polyembryony in non-apomictic citrus genotypes Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Adventitious embryony from nucellar cells is the mechanism leading to apomixis in Citrus sp. However, singular cases of polyembryony have been reported in non-apomictic genotypes as a consequence of 2x x 4x hybridizations and in vitro culture of isolated nucelli. The origin of the plants arising from the aforementioned processes remains unclear. The genetic structure (ploidy and allelic constitution with microsatellite markers) of plants obtained from polyembryonic seeds arising from 2x x 4x sexual hybridizations and those regenerated from nucellus culture in vitro was systematically analysed in different non-apomictic citrus genotypes. Histological studies were also conducted to try to identify the initiation process underlying polyembryony. All plants obtained from the same undeveloped seed in 2x x 4x hybridizations resulted from cleavage of the original zygotic embryo. Also, the plants obtained from in vitro nucellus culture were recovered by somatic embryogenesis from cells that shared the same genotype as the zygotic embryos of the same seed. It appears that in non-apomictic citrus genotypes, proembryos or embryogenic cells are formed by cleavage of the zygotic embryos and that the development of these adventitious embryos, normally hampered, can take place in vivo or in vitro as a result of two different mechanisms that prevent the dominance of the initial zygotic embryo. 2017-06-01T10:12:29Z 2017-06-01T10:12:29Z 2010 OCT article Aleza, P., Juarez, J., Ollitrault, P., Navarro, L. (2010). Polyembryony in non-apomictic citrus genotypes. Annals of Botany, 106(4), 533-545. 0305-7364 http://hdl.handle.net/20.500.11939/5510 10.1093/aob/mcq148 en openAccess Impreso |
| spellingShingle | Aleza, Pablo Juárez, José Ollitrault, Patrick Navarro, Luis Polyembryony in non-apomictic citrus genotypes |
| title | Polyembryony in non-apomictic citrus genotypes |
| title_full | Polyembryony in non-apomictic citrus genotypes |
| title_fullStr | Polyembryony in non-apomictic citrus genotypes |
| title_full_unstemmed | Polyembryony in non-apomictic citrus genotypes |
| title_short | Polyembryony in non-apomictic citrus genotypes |
| title_sort | polyembryony in non apomictic citrus genotypes |
| url | http://hdl.handle.net/20.500.11939/5510 |
| work_keys_str_mv | AT alezapablo polyembryonyinnonapomicticcitrusgenotypes AT juarezjose polyembryonyinnonapomicticcitrusgenotypes AT ollitraultpatrick polyembryonyinnonapomicticcitrusgenotypes AT navarroluis polyembryonyinnonapomicticcitrusgenotypes |