Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants
In Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase in 'Ca. L. asiaticus' occurrence. Gr...
| Main Authors: | , , , , , , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5507 |
| _version_ | 1855032289730232320 |
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| author | Lopes, S. A. Bertolini, Edson Frare, G. F. Martins, E. C. Wulff, N. A. Teixeira, D. C. Fernandes, N. G. Cambra, Mariano |
| author_browse | Bertolini, Edson Cambra, Mariano Fernandes, N. G. Frare, G. F. Lopes, S. A. Martins, E. C. Teixeira, D. C. Wulff, N. A. |
| author_facet | Lopes, S. A. Bertolini, Edson Frare, G. F. Martins, E. C. Wulff, N. A. Teixeira, D. C. Fernandes, N. G. Cambra, Mariano |
| author_sort | Lopes, S. A. |
| collection | ReDivia |
| description | In Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase in 'Ca. L. asiaticus' occurrence. Graft transmission experiments were used for a comparative study of both species using budsticks from symptomatic branches of field-affected trees as inoculum. The plants were inoculated with 'Ca. L. asiaticus' or 'Ca. L. americanus' alone, or simultaneously with both species. Symptom manifestation and conventional and quantitative real-time polymerase chain reaction were used for plant evaluations. 'Ca. L. americanus' was detected mainly in symptomatic plants and 'Ca. L. asiaticus' was detected in symptomatic plants as well as in infected plants prior to symptom manifestation. Transmission percentages varied from 54.7 to 88.0% for 'Ca. L. asiaticus' and 10.0 to 45.2% for 'Ca. L. americanus' in two experiments. In co-inoculated plants, 12.9% contained 'Ca. L. americanus' only, 40.3% contained 'Ca. L. asiaticus' only, and 19.3% contained both species. Average bacterial titers for 'Ca. L. asiaticus' and 'Ca. L. americanus', in log cells per gram of leaf midrib, were 6.42 and 4.87 for the experimental plants and 6.67 and 5.74 for the field trees used as the source of inoculum. The higher bacterial populations of the 'Ca. L. asiaticus'-infected plants provided an explanation for the disproportional increase in field prevalence of this species over time, based on the greater likelihood for pathogen transmission by the insect vector. |
| format | article |
| id | ReDivia5507 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55072025-04-25T14:43:09Z Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants Lopes, S. A. Bertolini, Edson Frare, G. F. Martins, E. C. Wulff, N. A. Teixeira, D. C. Fernandes, N. G. Cambra, Mariano In Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase in 'Ca. L. asiaticus' occurrence. Graft transmission experiments were used for a comparative study of both species using budsticks from symptomatic branches of field-affected trees as inoculum. The plants were inoculated with 'Ca. L. asiaticus' or 'Ca. L. americanus' alone, or simultaneously with both species. Symptom manifestation and conventional and quantitative real-time polymerase chain reaction were used for plant evaluations. 'Ca. L. americanus' was detected mainly in symptomatic plants and 'Ca. L. asiaticus' was detected in symptomatic plants as well as in infected plants prior to symptom manifestation. Transmission percentages varied from 54.7 to 88.0% for 'Ca. L. asiaticus' and 10.0 to 45.2% for 'Ca. L. americanus' in two experiments. In co-inoculated plants, 12.9% contained 'Ca. L. americanus' only, 40.3% contained 'Ca. L. asiaticus' only, and 19.3% contained both species. Average bacterial titers for 'Ca. L. asiaticus' and 'Ca. L. americanus', in log cells per gram of leaf midrib, were 6.42 and 4.87 for the experimental plants and 6.67 and 5.74 for the field trees used as the source of inoculum. The higher bacterial populations of the 'Ca. L. asiaticus'-infected plants provided an explanation for the disproportional increase in field prevalence of this species over time, based on the greater likelihood for pathogen transmission by the insect vector. 2017-06-01T10:12:28Z 2017-06-01T10:12:28Z 2009 MAR 2009 article Lopes, S. A., Bertolini, E., Frare, G. F., Martins, E. C., Wulff, N. A., Teixeira, D. C., Fernandes, N. G., Cambra, M. (2009). Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants. Phytopathology, 99(3), 301-306. 0031-949X http://hdl.handle.net/20.500.11939/5507 10.1094/PHYTO-99-3-0301 en openAccess Impreso |
| spellingShingle | Lopes, S. A. Bertolini, Edson Frare, G. F. Martins, E. C. Wulff, N. A. Teixeira, D. C. Fernandes, N. G. Cambra, Mariano Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title | Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title_full | Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title_fullStr | Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title_full_unstemmed | Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title_short | Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants |
| title_sort | graft transmission efficiencies and multiplication of candidatus liberibacter americanus and ca liberibacter asiaticus in citrus plants |
| url | http://hdl.handle.net/20.500.11939/5507 |
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