Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization
To better understand the molecular and physiological mechanisms underlying maintenance and release of seasonal bud dormancy in perennial trees, we identified differentially expressed genes during dormancy progression in reproductive buds from peach (Prunus persica [L.] Batsch) by suppression subtrac...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5479 |
| _version_ | 1855491931681849344 |
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| author | Leida, Carmen Terol, Javier Marti, Gracia Agustí, Manuel Llácer, Gerardo Badenes, María L. Ríos, Gabino |
| author_browse | Agustí, Manuel Badenes, María L. Leida, Carmen Llácer, Gerardo Marti, Gracia Ríos, Gabino Terol, Javier |
| author_facet | Leida, Carmen Terol, Javier Marti, Gracia Agustí, Manuel Llácer, Gerardo Badenes, María L. Ríos, Gabino |
| author_sort | Leida, Carmen |
| collection | ReDivia |
| description | To better understand the molecular and physiological mechanisms underlying maintenance and release of seasonal bud dormancy in perennial trees, we identified differentially expressed genes during dormancy progression in reproductive buds from peach (Prunus persica [L.] Batsch) by suppression subtractive hybridization (SSH) and microarray hybridization. Four SSH libraries were constructed, which were respectively enriched in cDNA highly expressed in dormant buds (named DR), in dormancy-released buds (RD) and in the cultivars with different chilling requirement, ‘Zincal 5’ (ZS) and ‘Springlady’ (SZ), sampled after dormancy release. About 2500 clones picked from the four libraries were loaded on a glass microarray. Hybridization of microarrays with the final products of SSH procedure was performed in order to validate the selected clones that were effectively enriched in their respective sample. Nearly 400 positive clones were sequenced, which corresponded to 101 different unigenes with diverse functional annotation. We obtained DAM4, 5 and 6 genes coding for MADS-box transcription factors previously related to growth cessation and terminal bud formation in the evergrowing mutant of peach. Several other cDNAs are similar to dormancy factors described in other species, and others have been related to bud dormancy for the first time in this study. Quantitative reverse transcription polymerase chain reaction analysis confirmed differential expression of cDNAs coding for a Zn-finger transcription factor, a GRAS-like regulator, a DNA-binding protein and proteins similar to forisome subunits involved in the reversible occlusion of sieve elements in Fabaceae, among others. |
| format | Artículo |
| id | ReDivia5479 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia54792025-04-25T14:42:28Z Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization Leida, Carmen Terol, Javier Marti, Gracia Agustí, Manuel Llácer, Gerardo Badenes, María L. Ríos, Gabino To better understand the molecular and physiological mechanisms underlying maintenance and release of seasonal bud dormancy in perennial trees, we identified differentially expressed genes during dormancy progression in reproductive buds from peach (Prunus persica [L.] Batsch) by suppression subtractive hybridization (SSH) and microarray hybridization. Four SSH libraries were constructed, which were respectively enriched in cDNA highly expressed in dormant buds (named DR), in dormancy-released buds (RD) and in the cultivars with different chilling requirement, ‘Zincal 5’ (ZS) and ‘Springlady’ (SZ), sampled after dormancy release. About 2500 clones picked from the four libraries were loaded on a glass microarray. Hybridization of microarrays with the final products of SSH procedure was performed in order to validate the selected clones that were effectively enriched in their respective sample. Nearly 400 positive clones were sequenced, which corresponded to 101 different unigenes with diverse functional annotation. We obtained DAM4, 5 and 6 genes coding for MADS-box transcription factors previously related to growth cessation and terminal bud formation in the evergrowing mutant of peach. Several other cDNAs are similar to dormancy factors described in other species, and others have been related to bud dormancy for the first time in this study. Quantitative reverse transcription polymerase chain reaction analysis confirmed differential expression of cDNAs coding for a Zn-finger transcription factor, a GRAS-like regulator, a DNA-binding protein and proteins similar to forisome subunits involved in the reversible occlusion of sieve elements in Fabaceae, among others. 2017-06-01T10:12:25Z 2017-06-01T10:12:25Z 2010 MAY 2010 article Leida, C., Terol, J., Marti, G., Agusti, M., Llacer, G., Badenes, M. L. et al. (2010). Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization. Tree physiology, 30(5), 655-666. 0829-318X http://hdl.handle.net/20.500.11939/5479 10.1093/treephys/tpq008 en openAccess Impreso |
| spellingShingle | Leida, Carmen Terol, Javier Marti, Gracia Agustí, Manuel Llácer, Gerardo Badenes, María L. Ríos, Gabino Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title | Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title_full | Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title_fullStr | Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title_full_unstemmed | Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title_short | Identification of genes associated with bud dormancy release in Prunus persica by suppression subtractive hybridization |
| title_sort | identification of genes associated with bud dormancy release in prunus persica by suppression subtractive hybridization |
| url | http://hdl.handle.net/20.500.11939/5479 |
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