Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner
Bud dormancy release in many woody perennial plants responds to the seasonal accumulation of chilling stimulus. MADS-box transcription factors encoded by DORMANCY ASSOCIATED MADS-box (DAM) genes in peach (Prunus persica) are implicated in this pathway, but other regulatory factors remain to be ident...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5474 |
| _version_ | 1855491931039072256 |
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| author | Leida, Carmen Conesa, Ana Llácer, Gerardo Badenes, María L. Ríos, Gabino |
| author_browse | Badenes, María L. Conesa, Ana Leida, Carmen Llácer, Gerardo Ríos, Gabino |
| author_facet | Leida, Carmen Conesa, Ana Llácer, Gerardo Badenes, María L. Ríos, Gabino |
| author_sort | Leida, Carmen |
| collection | ReDivia |
| description | Bud dormancy release in many woody perennial plants responds to the seasonal accumulation of chilling stimulus. MADS-box transcription factors encoded by DORMANCY ASSOCIATED MADS-box (DAM) genes in peach (Prunus persica) are implicated in this pathway, but other regulatory factors remain to be identified. In addition, the regulation of DAM gene expression is not well known at the molecular level. A microarray hybridization approach was performed to identify genes whose expression correlates with the bud dormancy-related behaviour in 10 different peach cultivars. Histone modifications in DAM6 gene were investigated by chromatin immunoprecipitation in two different cultivars. The expression of DAM4DAM6 and several genes related to abscisic acid and drought stress response correlated with the dormancy behaviour of peach cultivars. The trimethylation of histone H3 at K27 in the DAM6 promoter, coding region and the second large intron was preceded by a decrease in acetylated H3 and trimethylated H3K4 in the region of translation start, coinciding with repression of DAM6 during dormancy release. Analysis of chromatin modifications reinforced the role of epigenetic mechanisms in DAM6 regulation and bud dormancy release, and highlighted common features with the vernalization process in Arabidopsis thaliana and cereals. |
| format | Artículo |
| id | ReDivia5474 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia54742025-04-25T14:42:27Z Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner Leida, Carmen Conesa, Ana Llácer, Gerardo Badenes, María L. Ríos, Gabino Bud dormancy release in many woody perennial plants responds to the seasonal accumulation of chilling stimulus. MADS-box transcription factors encoded by DORMANCY ASSOCIATED MADS-box (DAM) genes in peach (Prunus persica) are implicated in this pathway, but other regulatory factors remain to be identified. In addition, the regulation of DAM gene expression is not well known at the molecular level. A microarray hybridization approach was performed to identify genes whose expression correlates with the bud dormancy-related behaviour in 10 different peach cultivars. Histone modifications in DAM6 gene were investigated by chromatin immunoprecipitation in two different cultivars. The expression of DAM4DAM6 and several genes related to abscisic acid and drought stress response correlated with the dormancy behaviour of peach cultivars. The trimethylation of histone H3 at K27 in the DAM6 promoter, coding region and the second large intron was preceded by a decrease in acetylated H3 and trimethylated H3K4 in the region of translation start, coinciding with repression of DAM6 during dormancy release. Analysis of chromatin modifications reinforced the role of epigenetic mechanisms in DAM6 regulation and bud dormancy release, and highlighted common features with the vernalization process in Arabidopsis thaliana and cereals. 2017-06-01T10:12:24Z 2017-06-01T10:12:24Z 2012 JAN 2012 article publishedVersion Leida, C., Conesa, A., Llacer, G., Badenes, M. L. & Rios, G. (2012). Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner. New Phytologist, 193(1), 67-80. 0028-646X http://hdl.handle.net/20.500.11939/5474 10.1111/j.1469-8137.2011.03863.x en openAccess Impreso |
| spellingShingle | Leida, Carmen Conesa, Ana Llácer, Gerardo Badenes, María L. Ríos, Gabino Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title | Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title_full | Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title_fullStr | Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title_full_unstemmed | Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title_short | Histone modifications and expression of DAM6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner |
| title_sort | histone modifications and expression of dam6 gene in peach are modulated during bud dormancy release in a cultivar dependent manner |
| url | http://hdl.handle.net/20.500.11939/5474 |
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