Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase
The citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. The recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induc...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5465 |
| _version_ | 1855032282610401280 |
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| author | Aleza, Pablo Juárez, José Cuenca, José Ollitrault, Patrick Navarro, Luis |
| author_browse | Aleza, Pablo Cuenca, José Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Aleza, Pablo Juárez, José Cuenca, José Ollitrault, Patrick Navarro, Luis |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | The citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. The recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. Triploid plants can be recovered by 2x x 4x sexual hybridization. In this work, we present an effective methodology to recover triploid plants from 2x x 4x hybridizations based on in vitro embryo rescue, ploidy level analysis by flow cytometry and genetic origin of triploid plants. The pollen viability of diploid and tetraploid citrus genotypes was analyzed by comparing the pollen germination rate in vitro. The pollen viability of tetraploid (doubled-diploid) genotypes is generally reduced but sufficient for successful pollination. Triploid embryos were identified in normal and undeveloped seeds that did not germinate under greenhouse conditions. The influence of parents and environmental conditions on obtaining triploid plants was analyzed and a strong interaction was noted between the parents and environmental conditions. The parental effect on the length of the juvenile phase was also demonstrated through observations of a large number of progeny over the last 15 years. The juvenile phase length of the triploid hybrids obtained with 'Fortune' mandarin as female parent and tetraploid 'Orlando' tangelo as male parent was shorter than the juvenile phase obtained with a clementine as female parent and tetraploids of 'Nova', 'W. Leaf' and 'Pineapple' male parents. Key message Effective methodology to recover citrus triploid plants from 2x x 4x sexual hybridizations and the parental effect on the length of the juvenile phase. |
| format | article |
| id | ReDivia5465 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia54652025-04-25T14:42:25Z Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase Aleza, Pablo Juárez, José Cuenca, José Ollitrault, Patrick Navarro, Luis The citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. The recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. Triploid plants can be recovered by 2x x 4x sexual hybridization. In this work, we present an effective methodology to recover triploid plants from 2x x 4x hybridizations based on in vitro embryo rescue, ploidy level analysis by flow cytometry and genetic origin of triploid plants. The pollen viability of diploid and tetraploid citrus genotypes was analyzed by comparing the pollen germination rate in vitro. The pollen viability of tetraploid (doubled-diploid) genotypes is generally reduced but sufficient for successful pollination. Triploid embryos were identified in normal and undeveloped seeds that did not germinate under greenhouse conditions. The influence of parents and environmental conditions on obtaining triploid plants was analyzed and a strong interaction was noted between the parents and environmental conditions. The parental effect on the length of the juvenile phase was also demonstrated through observations of a large number of progeny over the last 15 years. The juvenile phase length of the triploid hybrids obtained with 'Fortune' mandarin as female parent and tetraploid 'Orlando' tangelo as male parent was shorter than the juvenile phase obtained with a clementine as female parent and tetraploids of 'Nova', 'W. Leaf' and 'Pineapple' male parents. Key message Effective methodology to recover citrus triploid plants from 2x x 4x sexual hybridizations and the parental effect on the length of the juvenile phase. 2017-06-01T10:12:24Z 2017-06-01T10:12:24Z 2012 SEP article Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., & Navarro, L. (2012). Extensive citrus triploid hybrid production by 2x× 4x sexual hybridizations and parent-effect on the length of the juvenile phase. Plant cell reports, 31(9), 1723-1735. 0721-7714 http://hdl.handle.net/20.500.11939/5465 10.1007/s00299-012-1286-0 en openAccess Impreso |
| spellingShingle | Aleza, Pablo Juárez, José Cuenca, José Ollitrault, Patrick Navarro, Luis Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title | Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title_full | Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title_fullStr | Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title_full_unstemmed | Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title_short | Extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent-effect on the length of the juvenile phase |
| title_sort | extensive citrus triploid hybrid production by 2x x 4x sexual hybridizations and parent effect on the length of the juvenile phase |
| url | http://hdl.handle.net/20.500.11939/5465 |
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