Applications of Citrus Shoot-Tip Grafting In Vitro

The main application of shoot-tip grafting in vitro (STG) is to control graft-transmissible pathogens that requires the use of healthy trees in the new plantings. The routine application of STG using 0.1-0.2 mm shoot tips is very efficient for elimination of all citrus graft-transmissible pathogens...

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Autores principales: Juárez, José, Aleza, Pablo, Navarro, Luis
Otros Autores: Sabater-Munoz, Beatriz
Formato: Objeto de conferencia
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/5439
https://doi.org/10.17660/ActaHortic.2015.1065.79
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author Juárez, José
Aleza, Pablo
Navarro, Luis
author2 Sabater-Munoz, Beatriz
author_browse Aleza, Pablo
Juárez, José
Navarro, Luis
Sabater-Munoz, Beatriz
author_facet Sabater-Munoz, Beatriz
Juárez, José
Aleza, Pablo
Navarro, Luis
author_sort Juárez, José
collection ReDivia
description The main application of shoot-tip grafting in vitro (STG) is to control graft-transmissible pathogens that requires the use of healthy trees in the new plantings. The routine application of STG using 0.1-0.2 mm shoot tips is very efficient for elimination of all citrus graft-transmissible pathogens from local or imported varieties. It has allowed the recovery of hundreds of healthy cultivars and the planting of hundreds of millions of healthy certified trees worldwide. Only in Spain about 140 million certified nursery plants propagated from micrografted plants have been planted. STG is also a very useful technique for regeneration of elite genotypes in several research areas. In vitro grafting for these purposes may be done using larger shoots (up to 1 cm). STG is being routinely used for the following purposes: (i) Regeneration of somatic hybrids from embryos difficult to germinate; (ii) Regeneration of plants from irradiated shoots to produce seedless varieties; (iii) Regeneration of plants from haploid embryos that are very difficult to germinate. STG was used to regenerate the 'Clemenules' haploid plant that has been used by the International Citrus Genome Consortium to sequence the whole citrus genome; (iv) Production of stable tetraploid plants of non-apomictic genotypes, which are very useful for triploid breeding; (v) Regeneration of transgenic plants from shoots that are very difficult to root in vitro. STG has become a routine application in citrus genetic transformation.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia54392025-04-25T14:53:15Z Applications of Citrus Shoot-Tip Grafting In Vitro Acta Horticulturae Juárez, José Aleza, Pablo Navarro, Luis Sabater-Munoz, Beatriz The main application of shoot-tip grafting in vitro (STG) is to control graft-transmissible pathogens that requires the use of healthy trees in the new plantings. The routine application of STG using 0.1-0.2 mm shoot tips is very efficient for elimination of all citrus graft-transmissible pathogens from local or imported varieties. It has allowed the recovery of hundreds of healthy cultivars and the planting of hundreds of millions of healthy certified trees worldwide. Only in Spain about 140 million certified nursery plants propagated from micrografted plants have been planted. STG is also a very useful technique for regeneration of elite genotypes in several research areas. In vitro grafting for these purposes may be done using larger shoots (up to 1 cm). STG is being routinely used for the following purposes: (i) Regeneration of somatic hybrids from embryos difficult to germinate; (ii) Regeneration of plants from irradiated shoots to produce seedless varieties; (iii) Regeneration of plants from haploid embryos that are very difficult to germinate. STG was used to regenerate the 'Clemenules' haploid plant that has been used by the International Citrus Genome Consortium to sequence the whole citrus genome; (iv) Production of stable tetraploid plants of non-apomictic genotypes, which are very useful for triploid breeding; (v) Regeneration of transgenic plants from shoots that are very difficult to root in vitro. STG has become a routine application in citrus genetic transformation. 2017-06-01T10:12:22Z 2017-06-01T10:12:22Z 2015 2015 conferenceObject Juárez, J., Aleza, P., & Navarro, L. (2015). Applications of citrus shoot-tip grafting in vitro. Acta Horticulturae, 1065, 635-642. 0567-7572; 978-94-62610-53-8 http://hdl.handle.net/20.500.11939/5439 https://doi.org/10.17660/ActaHortic.2015.1065.79 en openAccess Impreso
spellingShingle Juárez, José
Aleza, Pablo
Navarro, Luis
Applications of Citrus Shoot-Tip Grafting In Vitro
title Applications of Citrus Shoot-Tip Grafting In Vitro
title_full Applications of Citrus Shoot-Tip Grafting In Vitro
title_fullStr Applications of Citrus Shoot-Tip Grafting In Vitro
title_full_unstemmed Applications of Citrus Shoot-Tip Grafting In Vitro
title_short Applications of Citrus Shoot-Tip Grafting In Vitro
title_sort applications of citrus shoot tip grafting in vitro
url http://hdl.handle.net/20.500.11939/5439
https://doi.org/10.17660/ActaHortic.2015.1065.79
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