Separation of Citrus Viroids by Shoot-Tip Grafting Invitro

Arizona 861‐S1 citron (Citrus media L.) infected with a severe exocortis isolate containing four citrus viroids was used as source of tissue for shoot‐tip grafting in vitro. Out of 51 attempts, 25 successful grafts were obtained. Only 16 plants survived transplanting and of these 12 were viroid‐free...

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Main Authors: Juárez, José, Molins, M., Navarro, Luis, Durán-Vila, Núria
Format: Artículo
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5438
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author Juárez, José
Molins, M.
Navarro, Luis
Durán-Vila, Núria
author_browse Durán-Vila, Núria
Juárez, José
Molins, M.
Navarro, Luis
author_facet Juárez, José
Molins, M.
Navarro, Luis
Durán-Vila, Núria
author_sort Juárez, José
collection ReDivia
description Arizona 861‐S1 citron (Citrus media L.) infected with a severe exocortis isolate containing four citrus viroids was used as source of tissue for shoot‐tip grafting in vitro. Out of 51 attempts, 25 successful grafts were obtained. Only 16 plants survived transplanting and of these 12 were viroid‐free. The viroid profile of the other four plants showed fewer viroids than the original field source. The significance of these results, as compared with previous reports on the recovery of viroid‐free plants, is discussed. The results show the usefulness of shoot‐tip grafting in vitro as a tool to recover single viroid isolates from complex field sources.
format Artículo
id ReDivia5438
institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
language Inglés
publishDate 2017
publishDateRange 2017
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spelling ReDivia54382025-04-25T14:42:20Z Separation of Citrus Viroids by Shoot-Tip Grafting Invitro Juárez, José Molins, M. Navarro, Luis Durán-Vila, Núria Arizona 861‐S1 citron (Citrus media L.) infected with a severe exocortis isolate containing four citrus viroids was used as source of tissue for shoot‐tip grafting in vitro. Out of 51 attempts, 25 successful grafts were obtained. Only 16 plants survived transplanting and of these 12 were viroid‐free. The viroid profile of the other four plants showed fewer viroids than the original field source. The significance of these results, as compared with previous reports on the recovery of viroid‐free plants, is discussed. The results show the usefulness of shoot‐tip grafting in vitro as a tool to recover single viroid isolates from complex field sources. 2017-06-01T10:12:21Z 2017-06-01T10:12:21Z 1990 SEP article Juárez, J., Molins, M., Navarro, L. & Durán-Vila, N. (1990). Separation of Citrus Viroids by Shoot-Tip Grafting Invitro. Plant Pathology, 39(3), 472-476. 0032-0862 http://hdl.handle.net/20.500.11939/5438 10.1111/j.1365-3059.1990.tb02523.x en openAccess Impreso
spellingShingle Juárez, José
Molins, M.
Navarro, Luis
Durán-Vila, Núria
Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title_full Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title_fullStr Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title_full_unstemmed Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title_short Separation of Citrus Viroids by Shoot-Tip Grafting Invitro
title_sort separation of citrus viroids by shoot tip grafting invitro
url http://hdl.handle.net/20.500.11939/5438
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