Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes
Background The success of an area-wide sterile insect technique (SIT) programme against Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) relies on the mating success of sterile males in the field. Limited information is available about the effectiveness of sterile males in achieving mates with...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5436 |
| _version_ | 1855032278215819264 |
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| author | Juan-Blasco, María Sabater-Munoz, Beatriz Argilés, Rafael Jaques, Josep A. Castanera, Pedro Urbaneja, Alberto |
| author_browse | Argilés, Rafael Castanera, Pedro Jaques, Josep A. Juan-Blasco, María Sabater-Munoz, Beatriz Urbaneja, Alberto |
| author_facet | Juan-Blasco, María Sabater-Munoz, Beatriz Argilés, Rafael Jaques, Josep A. Castanera, Pedro Urbaneja, Alberto |
| author_sort | Juan-Blasco, María |
| collection | ReDivia |
| description | Background The success of an area-wide sterile insect technique (SIT) programme against Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) relies on the mating success of sterile males in the field. Limited information is available about the effectiveness of sterile males in achieving mates with wild females and how these matings contribute to reducing wild populations. To this end, firstly a mating competition test was performed in the laboratory with different release ratios (1:1:0, 1:1:1, 1:1:5, 1:1:10 and 1:1:20 for wild females:wild males:sterile VIENNA-8 males respectively) and different host fruit. Secondly, the same release ratios were evaluated under semi-natural conditions on caged trees and on sentinel host. Results By means of molecular markers, VIENNA-8 male sperm was positively detected in those females exposed to the male ratios 1:5, 1:10 and 1:20 in the laboratory. In the field test, sterile VIENNA-8 male matings and the C. capitata progeny on apples were positively correlated with the ratio of sterile males released and with the percentage of sterile matings respectively. Conclusions These results confirm the validity of using the molecular detection of VIENNA-8 male sperm to predict the C. capitata population under semi-natural conditions. Implications of these results in measuring the efficacy of an SIT programme are discussed. (c) 2012 Society of Chemical Industry |
| format | article |
| id | ReDivia5436 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia54362025-04-25T14:42:20Z Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes Juan-Blasco, María Sabater-Munoz, Beatriz Argilés, Rafael Jaques, Josep A. Castanera, Pedro Urbaneja, Alberto Background The success of an area-wide sterile insect technique (SIT) programme against Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) relies on the mating success of sterile males in the field. Limited information is available about the effectiveness of sterile males in achieving mates with wild females and how these matings contribute to reducing wild populations. To this end, firstly a mating competition test was performed in the laboratory with different release ratios (1:1:0, 1:1:1, 1:1:5, 1:1:10 and 1:1:20 for wild females:wild males:sterile VIENNA-8 males respectively) and different host fruit. Secondly, the same release ratios were evaluated under semi-natural conditions on caged trees and on sentinel host. Results By means of molecular markers, VIENNA-8 male sperm was positively detected in those females exposed to the male ratios 1:5, 1:10 and 1:20 in the laboratory. In the field test, sterile VIENNA-8 male matings and the C. capitata progeny on apples were positively correlated with the ratio of sterile males released and with the percentage of sterile matings respectively. Conclusions These results confirm the validity of using the molecular detection of VIENNA-8 male sperm to predict the C. capitata population under semi-natural conditions. Implications of these results in measuring the efficacy of an SIT programme are discussed. (c) 2012 Society of Chemical Industry 2017-06-01T10:12:21Z 2017-06-01T10:12:21Z 2013 JUL 2013 article J.-Blasco, M., Sabater-Munoz, Beatriz, Argiles, Rafael, Jacas, J.A., Castanera, P., Urbaneja, A. (2013). Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes. Pest management science, 69(7), 857-864. 1526-498X http://hdl.handle.net/20.500.11939/5436 10.1002/ps.3448 en openAccess Impreso |
| spellingShingle | Juan-Blasco, María Sabater-Munoz, Beatriz Argilés, Rafael Jaques, Josep A. Castanera, Pedro Urbaneja, Alberto Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title | Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title_full | Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title_fullStr | Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title_full_unstemmed | Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title_short | Molecular tools for sterile sperm detection to monitor Ceratitis capitata populations under SIT programmes |
| title_sort | molecular tools for sterile sperm detection to monitor ceratitis capitata populations under sit programmes |
| url | http://hdl.handle.net/20.500.11939/5436 |
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