Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes
Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresist...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5413 |
| _version_ | 1855032275140345856 |
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| author | Jiménez-Trigos, E. Vicente, José Salvador Mocé, Eva Naturil-Alfonso, C. Fernandez-Gonzalez, R. Gutiérrez-Adan, A. Marco-Jiménez, Francisco |
| author_browse | Fernandez-Gonzalez, R. Gutiérrez-Adan, A. Jiménez-Trigos, E. Marco-Jiménez, Francisco Mocé, Eva Naturil-Alfonso, C. Vicente, José Salvador |
| author_facet | Jiménez-Trigos, E. Vicente, José Salvador Mocé, Eva Naturil-Alfonso, C. Fernandez-Gonzalez, R. Gutiérrez-Adan, A. Marco-Jiménez, Francisco |
| author_sort | Jiménez-Trigos, E. |
| collection | ReDivia |
| description | Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-beta-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1 mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes. |
| format | article |
| id | ReDivia5413 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia54132025-04-25T14:42:17Z Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes Jiménez-Trigos, E. Vicente, José Salvador Mocé, Eva Naturil-Alfonso, C. Fernandez-Gonzalez, R. Gutiérrez-Adan, A. Marco-Jiménez, Francisco Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-beta-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1 mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes. 2017-06-01T10:12:20Z 2017-06-01T10:12:20Z 2013 AUG 2013 article Jimenez-Trigos, E., Vicente, J.S., Moce, E., Naturil-Alfonso, C., Fernandez-Gonzalez, R., Gutierrez-Adan, A., Marco-Jimenez, F. (2013). Treatment with cholE.ol-loaded methyl-beta-cyclodextrin increased the cholE.ol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes. Cryobiology, 67(1), 106-108. 0011-2240 http://hdl.handle.net/20.500.11939/5413 10.1016/j.cryobiol.2013.05.006 en openAccess Impreso |
| spellingShingle | Jiménez-Trigos, E. Vicente, José Salvador Mocé, Eva Naturil-Alfonso, C. Fernandez-Gonzalez, R. Gutiérrez-Adan, A. Marco-Jiménez, Francisco Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title | Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title_full | Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title_fullStr | Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title_full_unstemmed | Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title_short | Treatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes |
| title_sort | treatment with cholesterol loaded methyl beta cyclodextrin increased the cholesterol in rabbit oocytes but did not improve developmental competence of cryopreserved oocytes |
| url | http://hdl.handle.net/20.500.11939/5413 |
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