In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals

During ripening, citrus fruit-peel undergoes 'colour break', a process characterized by the conversion of chloroplast to chromoplast. The process involves the progressive loss of chlorophylls and the gain of carotenoids, changing peel colour from green to orange. In the present work, the in...

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Main Authors: Iglesias, Domingo J., Tadeo, Francisco R., Legaz, Francisco, Primo-Millo, Eduardo, Talón, Manuel
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5368
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author Iglesias, Domingo J.
Tadeo, Francisco R.
Legaz, Francisco
Primo-Millo, Eduardo
Talón, Manuel
author_browse Iglesias, Domingo J.
Legaz, Francisco
Primo-Millo, Eduardo
Tadeo, Francisco R.
Talón, Manuel
author_facet Iglesias, Domingo J.
Tadeo, Francisco R.
Legaz, Francisco
Primo-Millo, Eduardo
Talón, Manuel
author_sort Iglesias, Domingo J.
collection ReDivia
description During ripening, citrus fruit-peel undergoes 'colour break', a process characterized by the conversion of chloroplast to chromoplast. The process involves the progressive loss of chlorophylls and the gain of carotenoids, changing peel colour from green to orange. In the present work, the in vivo and in vitro effects of supplemented nutrients (sucrose and nitrogen) and phytohormones (gibberellins \GA\ and ethylene) on colour change in fruit epicarp of Satsuma mandarin (Citrus unshiu (Mak,) Marc,, cv, Okitsu), were studied, The rate of colour break was correlated positively with sucrose content and negatively with nitrogen content. The removal of leaves blocked natural sucrose build-up and nitrogen reduction in the peel. Defoliation also inhibited chlorophyll disappearance and carotenoid accumulation, thereby preventing colour break. In vivo sucrose supplementation promoted sucrose accumulation and advanced colour break. In both in vivo and in vitro experiments, colour change promoted by sucrose was unaffected by ethylene but delayed by GA,. in non-supplemented plants, ethylene accelerated colour break while GA, had no detectable effects. Ethylene inhibitors effectively counteracted the sucrose effects on colour change. Collectively, these results suggest that the chloroplast to chromoplast conversion in citrus fruit epicarps is stimulated by sucrose accumulation, The sugar regulation appears to operate via ethylene, whereas GA may act as a repressor of the sucrose-ethylene stimulation.
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spelling ReDivia53682025-04-25T14:42:12Z In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals Iglesias, Domingo J. Tadeo, Francisco R. Legaz, Francisco Primo-Millo, Eduardo Talón, Manuel During ripening, citrus fruit-peel undergoes 'colour break', a process characterized by the conversion of chloroplast to chromoplast. The process involves the progressive loss of chlorophylls and the gain of carotenoids, changing peel colour from green to orange. In the present work, the in vivo and in vitro effects of supplemented nutrients (sucrose and nitrogen) and phytohormones (gibberellins \GA\ and ethylene) on colour change in fruit epicarp of Satsuma mandarin (Citrus unshiu (Mak,) Marc,, cv, Okitsu), were studied, The rate of colour break was correlated positively with sucrose content and negatively with nitrogen content. The removal of leaves blocked natural sucrose build-up and nitrogen reduction in the peel. Defoliation also inhibited chlorophyll disappearance and carotenoid accumulation, thereby preventing colour break. In vivo sucrose supplementation promoted sucrose accumulation and advanced colour break. In both in vivo and in vitro experiments, colour change promoted by sucrose was unaffected by ethylene but delayed by GA,. in non-supplemented plants, ethylene accelerated colour break while GA, had no detectable effects. Ethylene inhibitors effectively counteracted the sucrose effects on colour change. Collectively, these results suggest that the chloroplast to chromoplast conversion in citrus fruit epicarps is stimulated by sucrose accumulation, The sugar regulation appears to operate via ethylene, whereas GA may act as a repressor of the sucrose-ethylene stimulation. 2017-06-01T10:12:14Z 2017-06-01T10:12:14Z 2001 JUN 2001 article Iglesias, D. J., Tadeo, F.R., Legaz, F., Primo-Millo, E., Talón, M. (2001). In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals. Physiologia Plantarum, 112(2), 244-250. 0031-9317 http://hdl.handle.net/20.500.11939/5368 10.1034/j.1399-3054.2001.1120213.x en openAccess Impreso
spellingShingle Iglesias, Domingo J.
Tadeo, Francisco R.
Legaz, Francisco
Primo-Millo, Eduardo
Talón, Manuel
In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title_full In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title_fullStr In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title_full_unstemmed In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title_short In vivo sucrose stimulation of colour change in citrus fruit epicarps: Interactions between nutritional and hormonal signals
title_sort in vivo sucrose stimulation of colour change in citrus fruit epicarps interactions between nutritional and hormonal signals
url http://hdl.handle.net/20.500.11939/5368
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