Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences
Forty Erwinia amylovora strains isolated from the Middle Atlas Mountains in Morocco have been characterized using sequences from tandem repeat of variable numbers (VNTR) as chromosomal markers. Three VNTR sequences were identified and used for studying the genetic diversity of the strains using a PC...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5352 |
| _version_ | 1855491911449575424 |
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| author | Hannou, Najat Llop, Pablo Faure, Denis López, María M. Moumni, Mohieddine |
| author_browse | Faure, Denis Hannou, Najat Llop, Pablo López, María M. Moumni, Mohieddine |
| author_facet | Hannou, Najat Llop, Pablo Faure, Denis López, María M. Moumni, Mohieddine |
| author_sort | Hannou, Najat |
| collection | ReDivia |
| description | Forty Erwinia amylovora strains isolated from the Middle Atlas Mountains in Morocco have been characterized using sequences from tandem repeat of variable numbers (VNTR) as chromosomal markers. Three VNTR sequences were identified and used for studying the genetic diversity of the strains using a PCR fingerprinting method. Thirty nine strains showed high genetic homogeneity, but one strain displayed a profile quite different. We were able to obtain reproducible and characteristic profiles in strains from remote geographical areas, and associated them with each of the primers. Comparative analyses of Moroccan strains with three reference strains from Spain, France and England, were performed. The results revealed some polymorphisms compared with the English and the French strains, but notable similarities were observed with a Spanish strain obtained from plants imported from Belgium. The developed methodology has demonstrated its usefulness for being applied in epidemiological studies. |
| format | Artículo |
| id | ReDivia5352 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia53522025-04-25T14:42:09Z Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences Hannou, Najat Llop, Pablo Faure, Denis López, María M. Moumni, Mohieddine Forty Erwinia amylovora strains isolated from the Middle Atlas Mountains in Morocco have been characterized using sequences from tandem repeat of variable numbers (VNTR) as chromosomal markers. Three VNTR sequences were identified and used for studying the genetic diversity of the strains using a PCR fingerprinting method. Thirty nine strains showed high genetic homogeneity, but one strain displayed a profile quite different. We were able to obtain reproducible and characteristic profiles in strains from remote geographical areas, and associated them with each of the primers. Comparative analyses of Moroccan strains with three reference strains from Spain, France and England, were performed. The results revealed some polymorphisms compared with the English and the French strains, but notable similarities were observed with a Spanish strain obtained from plants imported from Belgium. The developed methodology has demonstrated its usefulness for being applied in epidemiological studies. 2017-06-01T10:12:12Z 2017-06-01T10:12:12Z 2013 AUG 2013 article Hannou, N., Llop, P., Faure, D., Lopez, M. M. & Moumni, M. (2013). Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences. European Journal of Plant Pathology, 136(4), 665-674. 0929-1873 http://hdl.handle.net/20.500.11939/5352 10.1007/s10658-013-0197-4 en openAccess Impreso |
| spellingShingle | Hannou, Najat Llop, Pablo Faure, Denis López, María M. Moumni, Mohieddine Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title | Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title_full | Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title_fullStr | Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title_full_unstemmed | Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title_short | Characterization of Erwinia amylovora strains from Middle Atlas Mountains in Morocco by PCR based on tandem repeat sequences |
| title_sort | characterization of erwinia amylovora strains from middle atlas mountains in morocco by pcr based on tandem repeat sequences |
| url | http://hdl.handle.net/20.500.11939/5352 |
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