Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers
Seven monoclonal antibodies (MAbs) to Erwinia carotovora subsp. atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I of E. carotovora subsp. atroseptica, the most common serogroup on potato tubers in different serological assays. Eighty-six strains belonging...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5329 |
| _version_ | 1855032261924093952 |
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| author | Gorris, María T. Alarcon, B. López, María M. Cambra, Mariano |
| author_browse | Alarcon, B. Cambra, Mariano Gorris, María T. López, María M. |
| author_facet | Gorris, María T. Alarcon, B. López, María M. Cambra, Mariano |
| author_sort | Gorris, María T. |
| collection | ReDivia |
| description | Seven monoclonal antibodies (MAbs) to Erwinia carotovora subsp. atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I of E. carotovora subsp. atroseptica, the most common serogroup on potato tubers in different serological assays. Eighty-six strains belonging to different E. carotovora subsp. atroseptica serogroups were assayed. Some strains of serogroup XXII also reacted positively. No cross-reactions were observed against other species of plant pathogenic bacteria or 162 saprophytic bacteria from potato tubers. Only one strain of E. chrysanthemi from potato cross-reacted. A comparison of several serological techniques to detect E. carotovora subsp. atroseptica on potato tubers was performed with MAb 4G4 or polyclonal antibodies. The organism was extracted directly from potato peels of artificially inoculated tubers by soaking or selective enrichment under anaerobiosis in a medium with polypectate. MAb 4G4 was able to detect specifically 240 E. carotovora subsp. atroseptica cells per mi by indirect immunofluorescence and immunofluorescence colony staining and after soaking by ELISA-DAS (double-antibody sandwich enzyme-linked immunosorbent assay) after enrichment. The same amount of cells was detected by using immunolectrotransfer with polyclonal antibodies, and E. carotovora subsp. atroseptica and subsp. carotovora were distinguished by the latter technique. ELISA-DAS using MAb 4G4 with an enrichment step also efficiently detected E. carotovora subsp. atroseptica in naturally infected tubers and plants. |
| format | article |
| id | ReDivia5329 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia53292025-04-25T14:42:04Z Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers Gorris, María T. Alarcon, B. López, María M. Cambra, Mariano Seven monoclonal antibodies (MAbs) to Erwinia carotovora subsp. atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I of E. carotovora subsp. atroseptica, the most common serogroup on potato tubers in different serological assays. Eighty-six strains belonging to different E. carotovora subsp. atroseptica serogroups were assayed. Some strains of serogroup XXII also reacted positively. No cross-reactions were observed against other species of plant pathogenic bacteria or 162 saprophytic bacteria from potato tubers. Only one strain of E. chrysanthemi from potato cross-reacted. A comparison of several serological techniques to detect E. carotovora subsp. atroseptica on potato tubers was performed with MAb 4G4 or polyclonal antibodies. The organism was extracted directly from potato peels of artificially inoculated tubers by soaking or selective enrichment under anaerobiosis in a medium with polypectate. MAb 4G4 was able to detect specifically 240 E. carotovora subsp. atroseptica cells per mi by indirect immunofluorescence and immunofluorescence colony staining and after soaking by ELISA-DAS (double-antibody sandwich enzyme-linked immunosorbent assay) after enrichment. The same amount of cells was detected by using immunolectrotransfer with polyclonal antibodies, and E. carotovora subsp. atroseptica and subsp. carotovora were distinguished by the latter technique. ELISA-DAS using MAb 4G4 with an enrichment step also efficiently detected E. carotovora subsp. atroseptica in naturally infected tubers and plants. 2017-06-01T10:12:09Z 2017-06-01T10:12:09Z 1994 JUN 1994 article Gorris, M. T., Alarcon, B., Lopez, M.M., Cambra, M. (1994). Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers. Applied and Environmental Microbiology, 60(6), 2076-2085. 0099-2240 http://hdl.handle.net/20.500.11939/5329 en openAccess Impreso |
| spellingShingle | Gorris, María T. Alarcon, B. López, María M. Cambra, Mariano Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title | Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title_full | Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title_fullStr | Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title_full_unstemmed | Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title_short | Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers |
| title_sort | characterization of monoclonal antibodies specific for erwinia carotovora subsp atroseptica and comparison of serological methods for its sensitive detection on potato tubers |
| url | http://hdl.handle.net/20.500.11939/5329 |
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