Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane
Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, Le. the encapsulation of explants in calcium alginate beads. Encapsulated explants are then precultured in liquid medium with a high sucrose concentration and partially desiccat...
| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5322 |
| _version_ | 1855032260653219840 |
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| author | Gonzalez-Arnao, Maria Teresa Engelmann, Florent |
| author_browse | Engelmann, Florent Gonzalez-Arnao, Maria Teresa |
| author_facet | Gonzalez-Arnao, Maria Teresa Engelmann, Florent |
| author_sort | Gonzalez-Arnao, Maria Teresa |
| collection | ReDivia |
| description | Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, Le. the encapsulation of explants in calcium alginate beads. Encapsulated explants are then precultured in liquid medium with a high sucrose concentration and partially desiccated before freezing. Encapsulating the explants allows the subsequent application of very drastic treatments including preculture with high sucrose concentrations and desiccation to low moisture contents which would be higly damaging or lethal to non-encapsulated samples. An encapsulation-dehydration protocol comprises the following steps: pretreatment, encapsulation, preculture, desiccation, freezing and storage, thawing and regrowth. Encapsulation-dehydration has been applied to around 40 different plant species. The optimization of the successive steps of the encapsulation-dehydration protocol is illustrated for sugarcane apices. |
| format | article |
| id | ReDivia5322 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia53222025-04-25T14:42:03Z Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane Gonzalez-Arnao, Maria Teresa Engelmann, Florent Encapsulation-dehydration is a cryopreservation technique based on the technology developed for producing synthetic seeds, Le. the encapsulation of explants in calcium alginate beads. Encapsulated explants are then precultured in liquid medium with a high sucrose concentration and partially desiccated before freezing. Encapsulating the explants allows the subsequent application of very drastic treatments including preculture with high sucrose concentrations and desiccation to low moisture contents which would be higly damaging or lethal to non-encapsulated samples. An encapsulation-dehydration protocol comprises the following steps: pretreatment, encapsulation, preculture, desiccation, freezing and storage, thawing and regrowth. Encapsulation-dehydration has been applied to around 40 different plant species. The optimization of the successive steps of the encapsulation-dehydration protocol is illustrated for sugarcane apices. 2017-06-01T10:12:08Z 2017-06-01T10:12:08Z 2006 MAY-JUN 2006 article Gonzalez-Arnao, M. Teresa, Engelmann, Florent (2006). Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane. Cryoletters, 27(3), 155-168. 0143-2044 http://hdl.handle.net/20.500.11939/5322 en openAccess Impreso |
| spellingShingle | Gonzalez-Arnao, Maria Teresa Engelmann, Florent Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title | Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title_full | Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title_fullStr | Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title_full_unstemmed | Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title_short | Cryopreservation of plant germplasm using the encapsulation-dehydration technique: Review and case study on sugarcane |
| title_sort | cryopreservation of plant germplasm using the encapsulation dehydration technique review and case study on sugarcane |
| url | http://hdl.handle.net/20.500.11939/5322 |
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