Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation
The aim of the present study was to evaluate the cryoprotectant effect of different non-permeating sugars for boar sperm. Pooled semen from three boars was used for the experiments. In the first experiment, the sperm quality of boar sperm cryopreserved with an egg-yolk based extender supplemented wi...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5308 |
| _version_ | 1855491903555895296 |
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| author | Gómez-Fernández, José Gómez-Izquierdo, Emilio Tomás, Cristina Mocé, Eva De-Mercado, Eduardo |
| author_browse | De-Mercado, Eduardo Gómez-Fernández, José Gómez-Izquierdo, Emilio Mocé, Eva Tomás, Cristina |
| author_facet | Gómez-Fernández, José Gómez-Izquierdo, Emilio Tomás, Cristina Mocé, Eva De-Mercado, Eduardo |
| author_sort | Gómez-Fernández, José |
| collection | ReDivia |
| description | The aim of the present study was to evaluate the cryoprotectant effect of different non-permeating sugars for boar sperm. Pooled semen from three boars was used for the experiments. In the first experiment, the sperm quality of boar sperm cryopreserved with an egg-yolk based extender supplemented with different monosaccharides (glucose, galactose or fructose) was compared to a control cryopreserved in lactose-egg yolk extender. In the second experiment, the effect of five disaccharides (lactose, sucrose, lactulose, trehalose or melibiose) on boar sperm cryosurvival was studied. Several sperm quality parameters were assessed by flow cytometry in samples incubated for 30 and 150 min at 37 degrees C after thawing: percentages of sperm with intact plasma membrane (SIPM), sperm presenting high plasma membrane fluidity (HPMF), sperm with intracellular reactive oxygen substances production (IROSP) and apoptotic sperm (AS). In addition, the percentages of total motile (TMS) and progressively motile sperm (PMS) were assessed at the same incubation times with a computer-assisted sperm analysis system. Freezing extenders supplemented with each of the monosaccharide presented smaller cryoprotective effect than the control extender supplemented with lactose (P < 0.05). However, from the three monosaccharides tested, glucose provided the best sperm quality after freezing-thawing. With respect to the disaccharides studied, samples frozen with the extender supplemented with lactulose exhibited in general the lowest sperm quality, except for the percentage of capacitated sperm, which was highest (P < 0.05) in the samples cryopreserved with the trehalose extender. Our results suggest that disaccharides have higher cryoprotective effect than monosaccharides, although the monosaccharide composition of the disaccharides is also important, since the best results were obtained with those disaccharides presenting glucose in their composition. (C) 2012 Elsevier B.V. All rights reserved. |
| format | Artículo |
| id | ReDivia5308 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia53082025-04-25T14:42:00Z Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation Gómez-Fernández, José Gómez-Izquierdo, Emilio Tomás, Cristina Mocé, Eva De-Mercado, Eduardo The aim of the present study was to evaluate the cryoprotectant effect of different non-permeating sugars for boar sperm. Pooled semen from three boars was used for the experiments. In the first experiment, the sperm quality of boar sperm cryopreserved with an egg-yolk based extender supplemented with different monosaccharides (glucose, galactose or fructose) was compared to a control cryopreserved in lactose-egg yolk extender. In the second experiment, the effect of five disaccharides (lactose, sucrose, lactulose, trehalose or melibiose) on boar sperm cryosurvival was studied. Several sperm quality parameters were assessed by flow cytometry in samples incubated for 30 and 150 min at 37 degrees C after thawing: percentages of sperm with intact plasma membrane (SIPM), sperm presenting high plasma membrane fluidity (HPMF), sperm with intracellular reactive oxygen substances production (IROSP) and apoptotic sperm (AS). In addition, the percentages of total motile (TMS) and progressively motile sperm (PMS) were assessed at the same incubation times with a computer-assisted sperm analysis system. Freezing extenders supplemented with each of the monosaccharide presented smaller cryoprotective effect than the control extender supplemented with lactose (P < 0.05). However, from the three monosaccharides tested, glucose provided the best sperm quality after freezing-thawing. With respect to the disaccharides studied, samples frozen with the extender supplemented with lactulose exhibited in general the lowest sperm quality, except for the percentage of capacitated sperm, which was highest (P < 0.05) in the samples cryopreserved with the trehalose extender. Our results suggest that disaccharides have higher cryoprotective effect than monosaccharides, although the monosaccharide composition of the disaccharides is also important, since the best results were obtained with those disaccharides presenting glucose in their composition. (C) 2012 Elsevier B.V. All rights reserved. 2017-06-01T10:12:06Z 2017-06-01T10:12:06Z 2012 JUL 2012 article Gomez-Fernandez, J., Gomez-Izquierdo, Emilio, Tomas, C., Moce, E., de Mercado, E. (2012). Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation. Animal Reproduction Science, 133(1-2), 109-116. 0378-4320 http://hdl.handle.net/20.500.11939/5308 10.1016/j.anireprosci.2012.06.010 en openAccess Impreso |
| spellingShingle | Gómez-Fernández, José Gómez-Izquierdo, Emilio Tomás, Cristina Mocé, Eva De-Mercado, Eduardo Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title | Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title_full | Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title_fullStr | Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title_full_unstemmed | Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title_short | Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| title_sort | effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation |
| url | http://hdl.handle.net/20.500.11939/5308 |
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