Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe
Grapevine Syrah virus-1 (GSyV-1) was identified by small-RNA deep sequencing in Slovak grapevine co-infected by several other viruses. The RT-PCR assays developed in this work substantially improved the virus detection and allowed the identification of GSyV-1 in tested grapevine samples from Slovaki...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5286 |
| _version_ | 1855491899392000000 |
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| author | Glasa, Miroslav Predajna, Lukas Soltys, Katarína Sabanadzovic, Sead Olmos, Antonio |
| author_browse | Glasa, Miroslav Olmos, Antonio Predajna, Lukas Sabanadzovic, Sead Soltys, Katarína |
| author_facet | Glasa, Miroslav Predajna, Lukas Soltys, Katarína Sabanadzovic, Sead Olmos, Antonio |
| author_sort | Glasa, Miroslav |
| collection | ReDivia |
| description | Grapevine Syrah virus-1 (GSyV-1) was identified by small-RNA deep sequencing in Slovak grapevine co-infected by several other viruses. The RT-PCR assays developed in this work substantially improved the virus detection and allowed the identification of GSyV-1 in tested grapevine samples from Slovakia and the Czech Republic at an unexpectedly high rate (ca. 30 %). Subsequently, complete genome sequences of 3 GSyV-1 isolates (2 Slovak and 1 Czech) were determined by Sanger sequencing, showing a typical marafivirus genome organization. Analyses of complete genome sequences showed a higher intra-group diversity among these 3 central European GSyV-1 isolates (differences reaching 7.1 % at the nucleotide level) in comparison to 3 previously characterized North American isolates (only 1.2 % intra-group divergence). A substantially higher divergence among central European isolates and their clustering into two major phylogenetic groups was further confirmed by the partial genome analysis of additional 26 isolates. The CP-centered study did not support the geography-based clustering among central European and American isolates. Nevertheless, the sequence data of the highly variable 5′-proximal portion of the genome obtained for few additional isolates from Slovakia and Czech Republic showed the presence of both, “European-” and “north American-like”, GSyV-1 isolates in the analyzed grapevine samples. |
| format | Artículo |
| id | ReDivia5286 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52862025-04-25T14:45:51Z Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe Glasa, Miroslav Predajna, Lukas Soltys, Katarína Sabanadzovic, Sead Olmos, Antonio Grapevine Syrah virus-1 (GSyV-1) was identified by small-RNA deep sequencing in Slovak grapevine co-infected by several other viruses. The RT-PCR assays developed in this work substantially improved the virus detection and allowed the identification of GSyV-1 in tested grapevine samples from Slovakia and the Czech Republic at an unexpectedly high rate (ca. 30 %). Subsequently, complete genome sequences of 3 GSyV-1 isolates (2 Slovak and 1 Czech) were determined by Sanger sequencing, showing a typical marafivirus genome organization. Analyses of complete genome sequences showed a higher intra-group diversity among these 3 central European GSyV-1 isolates (differences reaching 7.1 % at the nucleotide level) in comparison to 3 previously characterized North American isolates (only 1.2 % intra-group divergence). A substantially higher divergence among central European isolates and their clustering into two major phylogenetic groups was further confirmed by the partial genome analysis of additional 26 isolates. The CP-centered study did not support the geography-based clustering among central European and American isolates. Nevertheless, the sequence data of the highly variable 5′-proximal portion of the genome obtained for few additional isolates from Slovakia and Czech Republic showed the presence of both, “European-” and “north American-like”, GSyV-1 isolates in the analyzed grapevine samples. 2017-06-01T10:12:03Z 2017-06-01T10:12:03Z 2015 AUG 2015 article Glasa, Miroslav, Predajna, Lukas, Soltys, Katarina, Sabanadzovic, Sead, Olmos, A. (2015). Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe. Virus genes, 51(1), 112-121. 0920-8569 http://hdl.handle.net/20.500.11939/5286 10.1007/s11262-015-1201-1 en openAccess Impreso |
| spellingShingle | Glasa, Miroslav Predajna, Lukas Soltys, Katarína Sabanadzovic, Sead Olmos, Antonio Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title | Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title_full | Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title_fullStr | Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title_full_unstemmed | Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title_short | Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe |
| title_sort | detection and molecular characterisation of grapevine syrah virus 1 isolates from central europe |
| url | http://hdl.handle.net/20.500.11939/5286 |
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