Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumula...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5285 |
| _version_ | 1855491899186479104 |
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| author | Glasa, Miroslav Predajna, Lukas Kominek, Petr Nagyova, Alzbeta Candresse, Thierry Olmos, Antonio |
| author_browse | Candresse, Thierry Glasa, Miroslav Kominek, Petr Nagyova, Alzbeta Olmos, Antonio Predajna, Lukas |
| author_facet | Glasa, Miroslav Predajna, Lukas Kominek, Petr Nagyova, Alzbeta Candresse, Thierry Olmos, Antonio |
| author_sort | Glasa, Miroslav |
| collection | ReDivia |
| description | Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic. |
| format | Artículo |
| id | ReDivia5285 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52852025-04-25T14:45:51Z Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines Glasa, Miroslav Predajna, Lukas Kominek, Petr Nagyova, Alzbeta Candresse, Thierry Olmos, Antonio Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic. 2017-06-01T10:12:03Z 2017-06-01T10:12:03Z 2014 AUG 2014 article Glasa, Miroslav, Predajna, Lukas, Kominek, Petr, Nagyova, Alzbeta, Candresse, Thierry, Olmos, A. (2014). Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines. Archives of Virology, 159(8), 2103-2107. 0304-8608 http://hdl.handle.net/20.500.11939/5285 10.1007/s00705-014-2031-5 en openAccess Impreso |
| spellingShingle | Glasa, Miroslav Predajna, Lukas Kominek, Petr Nagyova, Alzbeta Candresse, Thierry Olmos, Antonio Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title | Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title_full | Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title_fullStr | Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title_full_unstemmed | Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title_short | Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines |
| title_sort | molecular characterization of divergent grapevine pinot gris virus isolates and their detection in slovak and czech grapevines |
| url | http://hdl.handle.net/20.500.11939/5285 |
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