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Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase

The expression of engineered single-chain variable fragments specific to the NIb RNA replicase of Plum pox virus (PPV) (scFv2A) in transgenic plants was successfully used as a strategy to interfere with viral infection. Different scFv2A fusion proteins were constructed to target those subcellular co...

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Main Authors: Gil, Maite, Esteban, Olga, Garcia, Juan Antonio, Pena, Leandro, Cambra, Mariano
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5281
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author Gil, Maite
Esteban, Olga
Garcia, Juan Antonio
Pena, Leandro
Cambra, Mariano
author_browse Cambra, Mariano
Esteban, Olga
Garcia, Juan Antonio
Gil, Maite
Pena, Leandro
author_facet Gil, Maite
Esteban, Olga
Garcia, Juan Antonio
Pena, Leandro
Cambra, Mariano
author_sort Gil, Maite
collection ReDivia
description The expression of engineered single-chain variable fragments specific to the NIb RNA replicase of Plum pox virus (PPV) (scFv2A) in transgenic plants was successfully used as a strategy to interfere with viral infection. Different scFv2A fusion proteins were constructed to target those subcellular compartments, such as the cytosol, endoplasmic reticulum (ER) membrane structures and the nucleus, where NIb protein presumably accumulates. Several transgenic lines of Nicotiana benthamiana plants expressing the scFv2A targeted to the cytosol (2A lines), ER (6K2 lines) and nucleus (NLS lines) were obtained. The protective effect of scFv expression was determined by mechanical virus inoculation in five 2A, three 6K2 and four NLS transgenic lines. The strongest resistance was afforded with the 2A-3 (six non-infected plants out of 10), 6K2-1 (17 out of 33) and NLS-11 (16 out of 19) transgenic lines. The success of this interference with PPV infection opens new possibilities for the control of this RNA virus and could be exploited not only to confer resistance in transgenic plants, but also to elucidate the role of the non-structural NIb protein in different cell compartments during viral infection.
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spelling ReDivia52812025-04-25T14:45:51Z Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase Gil, Maite Esteban, Olga Garcia, Juan Antonio Pena, Leandro Cambra, Mariano The expression of engineered single-chain variable fragments specific to the NIb RNA replicase of Plum pox virus (PPV) (scFv2A) in transgenic plants was successfully used as a strategy to interfere with viral infection. Different scFv2A fusion proteins were constructed to target those subcellular compartments, such as the cytosol, endoplasmic reticulum (ER) membrane structures and the nucleus, where NIb protein presumably accumulates. Several transgenic lines of Nicotiana benthamiana plants expressing the scFv2A targeted to the cytosol (2A lines), ER (6K2 lines) and nucleus (NLS lines) were obtained. The protective effect of scFv expression was determined by mechanical virus inoculation in five 2A, three 6K2 and four NLS transgenic lines. The strongest resistance was afforded with the 2A-3 (six non-infected plants out of 10), 6K2-1 (17 out of 33) and NLS-11 (16 out of 19) transgenic lines. The success of this interference with PPV infection opens new possibilities for the control of this RNA virus and could be exploited not only to confer resistance in transgenic plants, but also to elucidate the role of the non-structural NIb protein in different cell compartments during viral infection. 2017-06-01T10:12:03Z 2017-06-01T10:12:03Z 2011 OCT 2011 article Gil, M., Esteban, O., Garcia, J.A., Pena, L., Cambra, M. (2011). Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase. Plant Pathology, 60(5), 967-976. 0032-0862 http://hdl.handle.net/20.500.11939/5281 10.1111/j.1365-3059.2011.02448.x en openAccess Impreso
spellingShingle Gil, Maite
Esteban, Olga
Garcia, Juan Antonio
Pena, Leandro
Cambra, Mariano
Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title_full Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title_fullStr Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title_full_unstemmed Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title_short Resistance to Plum pox virus in plants expressing cytosolic and nuclear single-chain antibodies against the viral RNA NIb replicase
title_sort resistance to plum pox virus in plants expressing cytosolic and nuclear single chain antibodies against the viral rna nib replicase
url http://hdl.handle.net/20.500.11939/5281
work_keys_str_mv AT gilmaite resistancetoplumpoxvirusinplantsexpressingcytosolicandnuclearsinglechainantibodiesagainsttheviralrnanibreplicase
AT estebanolga resistancetoplumpoxvirusinplantsexpressingcytosolicandnuclearsinglechainantibodiesagainsttheviralrnanibreplicase
AT garciajuanantonio resistancetoplumpoxvirusinplantsexpressingcytosolicandnuclearsinglechainantibodiesagainsttheviralrnanibreplicase
AT penaleandro resistancetoplumpoxvirusinplantsexpressingcytosolicandnuclearsinglechainantibodiesagainsttheviralrnanibreplicase
AT cambramariano resistancetoplumpoxvirusinplantsexpressingcytosolicandnuclearsinglechainantibodiesagainsttheviralrnanibreplicase

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