Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures
Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cult...
| Main Authors: | , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5278 |
| _version_ | 1855032253005955072 |
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| author | Ghorbel, R. Navarro, Luis Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Ghorbel, R. Navarro, Luis |
| author_facet | Ghorbel, R. Navarro, Luis Durán-Vila, Núria |
| author_sort | Ghorbel, R. |
| collection | ReDivia |
| description | Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cultured in vitro depended on the CTV isolate and the plant host, and showed a correlation with the in vivo effects observed in biological indexing. Evaluation of the morphogenic response of stem segments of Mexican lime and grapefruit can be used as an additional tool for the biological characterization of CTV isolates. The symptoms on sweet orange plants obtained from regenerated shoots indicated that CTV is unevenly distributed in the host plant cells and that the regeneration process may be utilized as a tool to separate strains from complex field isolates. |
| format | article |
| id | ReDivia5278 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52782025-04-25T14:45:50Z Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures Ghorbel, R. Navarro, Luis Durán-Vila, Núria Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cultured in vitro depended on the CTV isolate and the plant host, and showed a correlation with the in vivo effects observed in biological indexing. Evaluation of the morphogenic response of stem segments of Mexican lime and grapefruit can be used as an additional tool for the biological characterization of CTV isolates. The symptoms on sweet orange plants obtained from regenerated shoots indicated that CTV is unevenly distributed in the host plant cells and that the regeneration process may be utilized as a tool to separate strains from complex field isolates. 2017-06-01T10:12:02Z 2017-06-01T10:12:02Z 1998 JUN article Ghorbel, R., Navarro, L., Duran-Vila, N. (1998). Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures. Plant Pathology, 47(3), 333-340. 0032-0862 http://hdl.handle.net/20.500.11939/5278 10.1046/j.1365-3059.1998.00236.x en openAccess Impreso |
| spellingShingle | Ghorbel, R. Navarro, Luis Durán-Vila, Núria Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title | Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title_full | Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title_fullStr | Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title_full_unstemmed | Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title_short | Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| title_sort | biological characterization of citrus tristeza virus isolates by in vitro tissue cultures |
| url | http://hdl.handle.net/20.500.11939/5278 |
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