Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains
Aim: To molecularly and phenotypically characterize a selection of Agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. Methods and Results: Most of the strains were biovar 3 (Agrobacterium vitis), and the remaining were atypical biovar 1...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5261 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.12791 |
| _version_ | 1855491894515073024 |
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| author | Genov, N. Llop, Pablo López, María M. Bobev, S. G. Álvarez, Belén |
| author_browse | Bobev, S. G. Genov, N. Llop, Pablo López, María M. Álvarez, Belén |
| author_facet | Genov, N. Llop, Pablo López, María M. Bobev, S. G. Álvarez, Belén |
| author_sort | Genov, N. |
| collection | ReDivia |
| description | Aim: To molecularly and phenotypically characterize a selection of Agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. Methods and Results: Most of the strains were biovar 3 (Agrobacterium vitis), and the remaining were atypical biovar 1 (Agrobacterium tumefaciens). All of them were tumourigenic on grapevine plants but differences in other hosts were observed. Chromosomal and plasmid-borne traits were analysed by gene amplification with four primer sets. Detection of the pectin enzyme hydrolase gene clearly distinguished A.vitis from the atypical A.tumefaciens. Regarding the virulence sensor gene, limited host range tumour-inducing plasmids were found in the atypical isolates. About opine utilization, most A.vitis and some A.tumefaciens contained octopine/cucumopine plasmids, but the nopaline-type was only detected in one A.tumefaciens. Conclusions: The A.vitis strains were molecularly and phenotypically more homogeneous than those of A.tumefaciens, the latter displaying some typical A.vitis characteristics, suggesting an adaptation to life in grapevine. Significance and Impact of the Study: The findings of this work will help to improve detection procedures of the pathogen, and demonstrate the pathogen diversity in cold vineyards, laying the groundwork for epidemiological studies and development of control strategies of the crown and cane gall disease. |
| format | Artículo |
| id | ReDivia5261 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52612025-04-25T14:45:47Z Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains Genov, N. Llop, Pablo López, María M. Bobev, S. G. Álvarez, Belén Aim: To molecularly and phenotypically characterize a selection of Agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. Methods and Results: Most of the strains were biovar 3 (Agrobacterium vitis), and the remaining were atypical biovar 1 (Agrobacterium tumefaciens). All of them were tumourigenic on grapevine plants but differences in other hosts were observed. Chromosomal and plasmid-borne traits were analysed by gene amplification with four primer sets. Detection of the pectin enzyme hydrolase gene clearly distinguished A.vitis from the atypical A.tumefaciens. Regarding the virulence sensor gene, limited host range tumour-inducing plasmids were found in the atypical isolates. About opine utilization, most A.vitis and some A.tumefaciens contained octopine/cucumopine plasmids, but the nopaline-type was only detected in one A.tumefaciens. Conclusions: The A.vitis strains were molecularly and phenotypically more homogeneous than those of A.tumefaciens, the latter displaying some typical A.vitis characteristics, suggesting an adaptation to life in grapevine. Significance and Impact of the Study: The findings of this work will help to improve detection procedures of the pathogen, and demonstrate the pathogen diversity in cold vineyards, laying the groundwork for epidemiological studies and development of control strategies of the crown and cane gall disease. 2017-06-01T10:12:00Z 2017-06-01T10:12:00Z 2015 JUN 2015 article Genov, N., Llop, P., Lopez, M. M., Bobev, S. G. & Alvarez, B. (2015). Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains. Journal of applied microbiology, 118(6), 1465-1477. 1364-5072 http://hdl.handle.net/20.500.11939/5261 10.1111/jam.12791 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.12791 en openAccess Impreso |
| spellingShingle | Genov, N. Llop, Pablo López, María M. Bobev, S. G. Álvarez, Belén Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title | Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title_full | Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title_fullStr | Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title_full_unstemmed | Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title_short | Molecular and phenotypic characterization of 'Agrobacterium' species from vineyards allows identification of typical 'Agrobacterium vitis' and atypical biovar 1 strains |
| title_sort | molecular and phenotypic characterization of agrobacterium species from vineyards allows identification of typical agrobacterium vitis and atypical biovar 1 strains |
| url | http://hdl.handle.net/20.500.11939/5261 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.12791 |
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