Viability of ICSI oocytes after caffeine treatment and sperm membrane removal with Triton X-100 in pigs

Non-adequate decondensation of injected sperm nucleus is one the main problems of intracytoplasmic sperm injection (ICSI) in porcine. With the aim of improving pronuclear formation, the effects on activation and embryo development rates of 0.1% Triton X-100 (TX) sperm pre-treatment for membrane remo...

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Bibliographic Details
Main Authors: García-Mengual, Elena, Garcia-Rosello, E., Alfonso, J., Salvador, I., Cebrián-Serrano, Alberto, Silvestre, Miguel A.
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5251

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