Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily.
UNLABELLED: ; PREMISE OF THE STUDY: Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to e...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5244 |
| _version_ | 1855032247694917632 |
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| author | García-Lor, Andrés Ancillo, Gema Navarro, Luis Ollitrault, Patrick |
| author_browse | Ancillo, Gema García-Lor, Andrés Navarro, Luis Ollitrault, Patrick |
| author_facet | García-Lor, Andrés Ancillo, Gema Navarro, Luis Ollitrault, Patrick |
| author_sort | García-Lor, Andrés |
| collection | ReDivia |
| description | UNLABELLED: ; PREMISE OF THE STUDY: Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to estimate the potential of SNP markers, selected from a limited intrageneric discovery panel, for ongoing broader diversity analysis at the intra- and intergeneric levels and systematic germplasm bank characterization. ; METHODS AND RESULTS: Forty-two SNP markers were developed using KASPar technology. Forty-one were successfully genotyped in all of the Citrus germplasm, where intra- and interspecific polymorphisms were observed. The transferability and diversity decreased with increasing taxonomic distance. ; CONCLUSIONS: SNP markers based on the KASPar method developed from sequence data of a limited intrageneric discovery panel provide a valuable molecular resource for genetic diversity analysis of germplasm within a genus and should be useful for germplasm fingerprinting at a much broader diversity level. |
| format | article |
| id | ReDivia5244 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52442025-04-25T14:45:45Z Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. García-Lor, Andrés Ancillo, Gema Navarro, Luis Ollitrault, Patrick Competitive Allele-Specific PCR Rutaceae genetic diversity single-nucleotide polymorphisms (SNPs) UNLABELLED: ; PREMISE OF THE STUDY: Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to estimate the potential of SNP markers, selected from a limited intrageneric discovery panel, for ongoing broader diversity analysis at the intra- and intergeneric levels and systematic germplasm bank characterization. ; METHODS AND RESULTS: Forty-two SNP markers were developed using KASPar technology. Forty-one were successfully genotyped in all of the Citrus germplasm, where intra- and interspecific polymorphisms were observed. The transferability and diversity decreased with increasing taxonomic distance. ; CONCLUSIONS: SNP markers based on the KASPar method developed from sequence data of a limited intrageneric discovery panel provide a valuable molecular resource for genetic diversity analysis of germplasm within a genus and should be useful for germplasm fingerprinting at a much broader diversity level. 2017-06-01T10:11:57Z 2017-06-01T10:11:57Z 2013 2013 Apr article publishedVersion Garcia-Lor, A., Ancillo, G., Navarro, L., Ollitrault, P. (2013). Citrus (Rutaceae), SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily.. Applications in plant sciences, 1(4), -. 2168-0450 http://hdl.handle.net/20.500.11939/5244 10.3732/apps.1200406 en openAccess Impreso |
| spellingShingle | Competitive Allele-Specific PCR Rutaceae genetic diversity single-nucleotide polymorphisms (SNPs) García-Lor, Andrés Ancillo, Gema Navarro, Luis Ollitrault, Patrick Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title | Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title_full | Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title_fullStr | Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title_full_unstemmed | Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title_short | Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily. |
| title_sort | citrus rutaceae snp markers based on competitive allele specific pcr transferability across the aurantioideae subfamily |
| topic | Competitive Allele-Specific PCR Rutaceae genetic diversity single-nucleotide polymorphisms (SNPs) |
| url | http://hdl.handle.net/20.500.11939/5244 |
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