Genetic analysis of apomixis in Citrus and Poncirus by molecular markers
Propagation of citrus rootstocks depends upon the production of clonal plants from nucellar seedlings. This makes apomixis one of the host important traits in breeding programs for citrus rootstocks. The genetic control of apomixis was studied in a 50-tree progeny derived from the cross C. volkameri...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5242 |
| _version_ | 1855491890666799104 |
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| author | Garcia, R. Asins, María J. Forner Valero, Juan B. Carbonell, Emilio A. |
| author_browse | Asins, María J. Carbonell, Emilio A. Forner Valero, Juan B. Garcia, R. |
| author_facet | Garcia, R. Asins, María J. Forner Valero, Juan B. Carbonell, Emilio A. |
| author_sort | Garcia, R. |
| collection | ReDivia |
| description | Propagation of citrus rootstocks depends upon the production of clonal plants from nucellar seedlings. This makes apomixis one of the host important traits in breeding programs for citrus rootstocks. The genetic control of apomixis was studied in a 50-tree progeny derived from the cross C. volkamerianaxP. trifoliata using 69 molecular markers and bulked segregant analysis. The proportion of nucellar seedlings was estimated by isoenzymatic analysis of 25 seedlings per tree for 2 consecutive years. The type of embryony (polyembryonic versus monoembryonic seeds) was also determined for fruit-yielding trees. Separate genetic maps for each parental species were developed. The integration and comparison of these maps could be accomplished using common multiallelic segregant loci. Differences in gene synteny between the two species-specific genetic maps were shown. Important distortions in the segregation of markers at several genomic regions, some of them also involving differences in the C-methylation pattern, have been observed, especially for the pollen parent. Analysis of quantitative trait loci (QTLs) revealed the presence of six genomic positions (two in P. trifoliata and four in C. volkameriana) contributing individually up to 24% of the total variation for apomixis. Within the same species, QTLs with positive and negative allele effects were present, even in the same linkage group. One of the markers associated to apomixis (Apo2) is also associated to embryony type. Therefore, the genetic control of apomictic reproduction found in citrus (nucellar embryony) is quite complex compared to what has been reported for gametophytic apomixis. Molecular markers linked to QTLs governing apomixis will be useful to assist selection of future apomictic rootstocks for citrus varieties. |
| format | Artículo |
| id | ReDivia5242 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52422025-04-25T14:45:44Z Genetic analysis of apomixis in Citrus and Poncirus by molecular markers Garcia, R. Asins, María J. Forner Valero, Juan B. Carbonell, Emilio A. Propagation of citrus rootstocks depends upon the production of clonal plants from nucellar seedlings. This makes apomixis one of the host important traits in breeding programs for citrus rootstocks. The genetic control of apomixis was studied in a 50-tree progeny derived from the cross C. volkamerianaxP. trifoliata using 69 molecular markers and bulked segregant analysis. The proportion of nucellar seedlings was estimated by isoenzymatic analysis of 25 seedlings per tree for 2 consecutive years. The type of embryony (polyembryonic versus monoembryonic seeds) was also determined for fruit-yielding trees. Separate genetic maps for each parental species were developed. The integration and comparison of these maps could be accomplished using common multiallelic segregant loci. Differences in gene synteny between the two species-specific genetic maps were shown. Important distortions in the segregation of markers at several genomic regions, some of them also involving differences in the C-methylation pattern, have been observed, especially for the pollen parent. Analysis of quantitative trait loci (QTLs) revealed the presence of six genomic positions (two in P. trifoliata and four in C. volkameriana) contributing individually up to 24% of the total variation for apomixis. Within the same species, QTLs with positive and negative allele effects were present, even in the same linkage group. One of the markers associated to apomixis (Apo2) is also associated to embryony type. Therefore, the genetic control of apomictic reproduction found in citrus (nucellar embryony) is quite complex compared to what has been reported for gametophytic apomixis. Molecular markers linked to QTLs governing apomixis will be useful to assist selection of future apomictic rootstocks for citrus varieties. 2017-06-01T10:11:57Z 2017-06-01T10:11:57Z 1999 AUG 1999 article Garcia, R., Asins, M.J., Forner, J., Carbonell, E.A. (1999). Genetic analysis of apomixis in Citrus and Poncirus by molecular markers. Theoretical and Applied Genetics, 99(3-4), 511-518. 0040-5752 http://hdl.handle.net/20.500.11939/5242 10.1007/s001220051264 en openAccess Impreso |
| spellingShingle | Garcia, R. Asins, María J. Forner Valero, Juan B. Carbonell, Emilio A. Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title | Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title_full | Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title_fullStr | Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title_full_unstemmed | Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title_short | Genetic analysis of apomixis in Citrus and Poncirus by molecular markers |
| title_sort | genetic analysis of apomixis in citrus and poncirus by molecular markers |
| url | http://hdl.handle.net/20.500.11939/5242 |
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