Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato
The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/helicase domain in...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5228 |
| _version_ | 1855032245630271488 |
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| author | Galipienso, Luis Rubio, Luis Lopez, Carmelo Soler, Salvador Aramburu, Jose |
| author_browse | Aramburu, Jose Galipienso, Luis Lopez, Carmelo Rubio, Luis Soler, Salvador |
| author_facet | Galipienso, Luis Rubio, Luis Lopez, Carmelo Soler, Salvador Aramburu, Jose |
| author_sort | Galipienso, Luis |
| collection | ReDivia |
| description | The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with alpha-helix and beta-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome. |
| format | article |
| id | ReDivia5228 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52282025-04-25T14:45:42Z Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato Galipienso, Luis Rubio, Luis Lopez, Carmelo Soler, Salvador Aramburu, Jose The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with alpha-helix and beta-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome. 2017-06-01T10:11:55Z 2017-06-01T10:11:55Z 2009 OCT 2009 article Galipienso, L., Rubio, L., Lopez, Carmelo, Soler, Salvador, Aramburu, J. (2009). Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato. Virus genes, 39(2), 256-260. 0920-8569 http://hdl.handle.net/20.500.11939/5228 10.1007/s11262-009-0388-4 en openAccess Impreso |
| spellingShingle | Galipienso, Luis Rubio, Luis Lopez, Carmelo Soler, Salvador Aramburu, Jose Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title | Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title_full | Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title_fullStr | Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title_full_unstemmed | Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title_short | Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato |
| title_sort | complete nucleotide sequence of a spanish isolate of parietaria mottle virus infecting tomato |
| url | http://hdl.handle.net/20.500.11939/5228 |
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