Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange
The genetic variability and evolution of parietaria mottle virus (PMoV) of the genus Ilarvirus was studied by analyzing nucleotide sequences of 2b and CP genes from isolates collected in different countries. Phylogenetic analysis showed that PMoV isolates clustered in different clades: one (clade I)...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5225 |
| _version_ | 1855032245274804224 |
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| author | Galipienso, Luis Martínez, Carolina Willemsen, Anouk Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Rubio, Luis |
| author_browse | Alfaro-Fernández, Ana Davino, Salvatore Font-San-Ambrosio, Isabel Galipienso, Luis Martínez, Carolina Rubio, Luis Willemsen, Anouk |
| author_facet | Galipienso, Luis Martínez, Carolina Willemsen, Anouk Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Rubio, Luis |
| author_sort | Galipienso, Luis |
| collection | ReDivia |
| description | The genetic variability and evolution of parietaria mottle virus (PMoV) of the genus Ilarvirus was studied by analyzing nucleotide sequences of 2b and CP genes from isolates collected in different countries. Phylogenetic analysis showed that PMoV isolates clustered in different clades: one (clade I) composed of only Italian isolates and three clades (clades II-IV) including the Spanish isolates. The Greek isolate GrT-1 used in this study was in clade IV for the CP phylogenetic tree whereas it formed a separate branch in the 2b phylogenetic tree. The nucleotide sequence diversity of both the 2b and CP genes was low (0.062 +/- A 0.006 and 0.063 +/- A 0.006 for 2b and CP, respectively) but higher than those of other ilarviruses. Distribution of synonymous and nonsynonymous substitutions revealed that 2b and CP proteins are under purifying selection, with some positions under diversifying selection. Genetic exchange among Spanish isolates was also detected. |
| format | article |
| id | ReDivia5225 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52252025-04-25T14:45:42Z Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange Galipienso, Luis Martínez, Carolina Willemsen, Anouk Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Rubio, Luis The genetic variability and evolution of parietaria mottle virus (PMoV) of the genus Ilarvirus was studied by analyzing nucleotide sequences of 2b and CP genes from isolates collected in different countries. Phylogenetic analysis showed that PMoV isolates clustered in different clades: one (clade I) composed of only Italian isolates and three clades (clades II-IV) including the Spanish isolates. The Greek isolate GrT-1 used in this study was in clade IV for the CP phylogenetic tree whereas it formed a separate branch in the 2b phylogenetic tree. The nucleotide sequence diversity of both the 2b and CP genes was low (0.062 +/- A 0.006 and 0.063 +/- A 0.006 for 2b and CP, respectively) but higher than those of other ilarviruses. Distribution of synonymous and nonsynonymous substitutions revealed that 2b and CP proteins are under purifying selection, with some positions under diversifying selection. Genetic exchange among Spanish isolates was also detected. 2017-06-01T10:11:55Z 2017-06-01T10:11:55Z 2015 OCT 2015 article Galipienso, L., Martinez, Carolina, Willemsen, Anouk, Alfaro-Fernandez, Ana, Font-San Ambrosio, I., Davino, Salvatore, Rubio, L. (2015). Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange. Archives of Virology, 160(10), 2611-2616. 0304-8608 http://hdl.handle.net/20.500.11939/5225 10.1007/s00705-015-2550-8 en openAccess Impreso |
| spellingShingle | Galipienso, Luis Martínez, Carolina Willemsen, Anouk Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Rubio, Luis Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title | Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title_full | Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title_fullStr | Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title_full_unstemmed | Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title_short | Genetic variability and evolutionary analysis of parietaria mottle virus: role of selection and genetic exchange |
| title_sort | genetic variability and evolutionary analysis of parietaria mottle virus role of selection and genetic exchange |
| url | http://hdl.handle.net/20.500.11939/5225 |
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