Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars
Since its outbreak in Spain during the fall of 2000, Cucumber vein yellowing virus (CVYV) has become into a limiting factor in cucurbit cultivation in this country, particularly in cucumber. The impact of the virus has been limited after the introduction of resistant cucumber cultivars and the imple...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5220 |
| _version_ | 1855491887807332352 |
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| author | Galipienso, Luis Janssen, Dirk Rubio, Luis Aramburu, Jose Velasco, Leonardo |
| author_browse | Aramburu, Jose Galipienso, Luis Janssen, Dirk Rubio, Luis Velasco, Leonardo |
| author_facet | Galipienso, Luis Janssen, Dirk Rubio, Luis Aramburu, Jose Velasco, Leonardo |
| author_sort | Galipienso, Luis |
| collection | ReDivia |
| description | Since its outbreak in Spain during the fall of 2000, Cucumber vein yellowing virus (CVYV) has become into a limiting factor in cucurbit cultivation in this country, particularly in cucumber. The impact of the virus has been limited after the introduction of resistant cucumber cultivars and the implementation of cultural practices to control whitefly, the virus vector. In Spain, CVYV isolates are genetically similar and cucumber resistance breaking isolates have not been reported. A CVYV isolate from Jordan (Jor) was previously reported as being more virulent in cucumber cv. Beit Alfa than an isolate from Israel. To study the reactions of different cucumber cultivars to the virulent Jor isolate in comparison with a Spanish isolate of (Alm 1A), we have inoculated both isolates in 10 commercial cucumber cultivars with resistance to the virus. Also, we have used real-time RT-PCR to estimate virus accumulation of Jor and Alm 1A isolates in three of these cultivars, one described as susceptible and two as resistant to CVYV. The Spanish Alm 1A isolate caused mild or no symptoms in resistant cultivars whereas Jor isolate caused intense symptoms in all cultivars. Alm 1A genomic RNA accumulated similarly in the three cultivars, whereas the accumulation of Jor genomic RNA was lower in the resistant cultivars than in the susceptible cultivar. Also the Jor isolate and accumulated earlier and at a higher level than the Alm 1A isolate in the three cultivars. (C) 2012 Elsevier Ltd. All rights reserved. |
| format | Artículo |
| id | ReDivia5220 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52202025-04-25T14:45:41Z Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars Galipienso, Luis Janssen, Dirk Rubio, Luis Aramburu, Jose Velasco, Leonardo Since its outbreak in Spain during the fall of 2000, Cucumber vein yellowing virus (CVYV) has become into a limiting factor in cucurbit cultivation in this country, particularly in cucumber. The impact of the virus has been limited after the introduction of resistant cucumber cultivars and the implementation of cultural practices to control whitefly, the virus vector. In Spain, CVYV isolates are genetically similar and cucumber resistance breaking isolates have not been reported. A CVYV isolate from Jordan (Jor) was previously reported as being more virulent in cucumber cv. Beit Alfa than an isolate from Israel. To study the reactions of different cucumber cultivars to the virulent Jor isolate in comparison with a Spanish isolate of (Alm 1A), we have inoculated both isolates in 10 commercial cucumber cultivars with resistance to the virus. Also, we have used real-time RT-PCR to estimate virus accumulation of Jor and Alm 1A isolates in three of these cultivars, one described as susceptible and two as resistant to CVYV. The Spanish Alm 1A isolate caused mild or no symptoms in resistant cultivars whereas Jor isolate caused intense symptoms in all cultivars. Alm 1A genomic RNA accumulated similarly in the three cultivars, whereas the accumulation of Jor genomic RNA was lower in the resistant cultivars than in the susceptible cultivar. Also the Jor isolate and accumulated earlier and at a higher level than the Alm 1A isolate in the three cultivars. (C) 2012 Elsevier Ltd. All rights reserved. 2017-06-01T10:11:54Z 2017-06-01T10:11:54Z 2013 JAN 2013 article Galipienso, L., Janssen, D., Rubio, L., Aramburu, J., Velasco, L. (2013). Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars. Crop Protection, 43, 141-145. 0261-2194 http://hdl.handle.net/20.500.11939/5220 10.1016/j.cropro.2012.08.004 en openAccess Impreso |
| spellingShingle | Galipienso, Luis Janssen, Dirk Rubio, Luis Aramburu, Jose Velasco, Leonardo Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title | Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title_full | Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title_fullStr | Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title_full_unstemmed | Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title_short | Cucumber vein yellowing virus isolate-specific expression of symptoms and viral RNA accumulation in susceptible and resistant cucumber cultivars |
| title_sort | cucumber vein yellowing virus isolate specific expression of symptoms and viral rna accumulation in susceptible and resistant cucumber cultivars |
| url | http://hdl.handle.net/20.500.11939/5220 |
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