Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain
The population structure and genetic variation of two begomoviruses: tomato yellow leaf curl Sardinia virus (TYLCSV) and tomato yellow leaf curl virus (TYLCV) in tomato crops of Spain were studied from 1997 until 2001. Restriction digestion of a genomic region comprised of the CP coat protein gene (...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5206 |
| _version_ | 1855032242367102976 |
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| author | Font-San-Ambrosio, Isabel Rubio, Luis Martinez-Culebras, Pedro Vicente Jordá, Concepción |
| author_browse | Font-San-Ambrosio, Isabel Jordá, Concepción Martinez-Culebras, Pedro Vicente Rubio, Luis |
| author_facet | Font-San-Ambrosio, Isabel Rubio, Luis Martinez-Culebras, Pedro Vicente Jordá, Concepción |
| author_sort | Font-San-Ambrosio, Isabel |
| collection | ReDivia |
| description | The population structure and genetic variation of two begomoviruses: tomato yellow leaf curl Sardinia virus (TYLCSV) and tomato yellow leaf curl virus (TYLCV) in tomato crops of Spain were studied from 1997 until 2001. Restriction digestion of a genomic region comprised of the CP coat protein gene (CPR) of 358 TYLC virus isolates enabled us to classify them into 14 haplotypes. Nucleotide sequences of two genomic regions: CPR, and the surrounding intergenic region (SIR) were determined for at least two isolates per haplotype. SIR was more variable than CPR and showed multiple recombination events whereas no recombination was detected within CPR. In all geographic regions except Murcia, the population was, or evolved to be composed of one predominant haplotype with a low genetic diversity (<0.0180). In Murcia, two successive changes of the predominant haplotype were observed in the best studied population. Phylogenetic analysis showed that the TYLCSV sequences determined clustered with sequences obtained from the GenBank of other TYLCSV Spanish isolates which were clearly separated from TYLCSV Italian isolates. Most of our TYLCV sequences were similar to those of isolates from Japan and Portugal, and the sequences obtained from TYLCV isolates from the Canary island of Lanzarote were similar to those of Caribbean TYLCV isolates. |
| format | article |
| id | ReDivia5206 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52062025-04-25T14:45:39Z Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain Font-San-Ambrosio, Isabel Rubio, Luis Martinez-Culebras, Pedro Vicente Jordá, Concepción The population structure and genetic variation of two begomoviruses: tomato yellow leaf curl Sardinia virus (TYLCSV) and tomato yellow leaf curl virus (TYLCV) in tomato crops of Spain were studied from 1997 until 2001. Restriction digestion of a genomic region comprised of the CP coat protein gene (CPR) of 358 TYLC virus isolates enabled us to classify them into 14 haplotypes. Nucleotide sequences of two genomic regions: CPR, and the surrounding intergenic region (SIR) were determined for at least two isolates per haplotype. SIR was more variable than CPR and showed multiple recombination events whereas no recombination was detected within CPR. In all geographic regions except Murcia, the population was, or evolved to be composed of one predominant haplotype with a low genetic diversity (<0.0180). In Murcia, two successive changes of the predominant haplotype were observed in the best studied population. Phylogenetic analysis showed that the TYLCSV sequences determined clustered with sequences obtained from the GenBank of other TYLCSV Spanish isolates which were clearly separated from TYLCSV Italian isolates. Most of our TYLCV sequences were similar to those of isolates from Japan and Portugal, and the sequences obtained from TYLCV isolates from the Canary island of Lanzarote were similar to those of Caribbean TYLCV isolates. 2017-06-01T10:11:53Z 2017-06-01T10:11:53Z 2007 SEP 2007 article Font, M. I., Rubio, L., Martinez-Culebras, P. Vicente, Jorda, C. (2007). Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain. Virus research, 128(1-2), 43-51. 0168-1702 http://hdl.handle.net/20.500.11939/5206 10.1016/j.virusres.2007.04.003 en openAccess Impreso |
| spellingShingle | Font-San-Ambrosio, Isabel Rubio, Luis Martinez-Culebras, Pedro Vicente Jordá, Concepción Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title | Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title_full | Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title_fullStr | Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title_full_unstemmed | Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title_short | Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain |
| title_sort | genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in spain |
| url | http://hdl.handle.net/20.500.11939/5206 |
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