Citrus tristeza virus p23: a unique protein mediating key virus-host interactions
The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3′-terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an abundant subgenomic RNA. p23, an RNA-binding protein with a putative zinc-finger domain and some basi...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5205 |
| _version_ | 1855032242185699328 |
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| author | Flores, Ricardo Ruiz-Ruiz, Susana Soler, Nuria Sánchez-Navarro, Jesús A. Fagoaga, Carmen Lopez, Carmelo Navarro, Luis Moreno, Pedro Pena, Leandro |
| author_browse | Fagoaga, Carmen Flores, Ricardo Lopez, Carmelo Moreno, Pedro Navarro, Luis Pena, Leandro Ruiz-Ruiz, Susana Soler, Nuria Sánchez-Navarro, Jesús A. |
| author_facet | Flores, Ricardo Ruiz-Ruiz, Susana Soler, Nuria Sánchez-Navarro, Jesús A. Fagoaga, Carmen Lopez, Carmelo Navarro, Luis Moreno, Pedro Pena, Leandro |
| author_sort | Flores, Ricardo |
| collection | ReDivia |
| description | The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3′-terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an abundant subgenomic RNA. p23, an RNA-binding protein with a putative zinc-finger domain and some basic motifs, is unique to CTV because no homologs have been found in other closteroviruses, including the type species of the genus Beet yellows virus (despite both viruses having many homologous genes). Consequently, p23 might have evolved for the specific interaction of CTV with its citrus hosts. From a functional perspective p23 has been involved in many roles: (I) regulation of the asymmetrical accumulation of CTV RNA strands, (II) induction of the seedling yellows syndrome in sour orange and grapefruit, (III) intracellular suppression of RNA silencing, (IV) elicitation of CTV-like symptoms when expressed ectopically as a transgene in several Citrus spp., and (V) enhancement of systemic infection (and virus accumulation) in sour orange and CTV release from the phloem in p23-expressing transgenic sweet and sour orange. Moreover, transformation of Mexican lime with intron-hairpin constructs designed for the co-inactivation of p23 and the two other CTV silencing suppressors results in complete resistance against the homologous virus. From a cellular point of view, recent data indicate that p23 accumulates preferentially in the nucleolus, being the first closterovirus protein with such a subcellular localization, as well as in plasmodesmata. These major accumulation sites most likely determine some of the functional roles of p23. |
| format | article |
| id | ReDivia5205 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52052025-04-25T14:45:38Z Citrus tristeza virus p23: a unique protein mediating key virus-host interactions Flores, Ricardo Ruiz-Ruiz, Susana Soler, Nuria Sánchez-Navarro, Jesús A. Fagoaga, Carmen Lopez, Carmelo Navarro, Luis Moreno, Pedro Pena, Leandro The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3′-terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an abundant subgenomic RNA. p23, an RNA-binding protein with a putative zinc-finger domain and some basic motifs, is unique to CTV because no homologs have been found in other closteroviruses, including the type species of the genus Beet yellows virus (despite both viruses having many homologous genes). Consequently, p23 might have evolved for the specific interaction of CTV with its citrus hosts. From a functional perspective p23 has been involved in many roles: (I) regulation of the asymmetrical accumulation of CTV RNA strands, (II) induction of the seedling yellows syndrome in sour orange and grapefruit, (III) intracellular suppression of RNA silencing, (IV) elicitation of CTV-like symptoms when expressed ectopically as a transgene in several Citrus spp., and (V) enhancement of systemic infection (and virus accumulation) in sour orange and CTV release from the phloem in p23-expressing transgenic sweet and sour orange. Moreover, transformation of Mexican lime with intron-hairpin constructs designed for the co-inactivation of p23 and the two other CTV silencing suppressors results in complete resistance against the homologous virus. From a cellular point of view, recent data indicate that p23 accumulates preferentially in the nucleolus, being the first closterovirus protein with such a subcellular localization, as well as in plasmodesmata. These major accumulation sites most likely determine some of the functional roles of p23. 2017-06-01T10:11:53Z 2017-06-01T10:11:53Z 2013 MAY 3 article publishedVersion Flores, R., Ruiz-Ruiz, S., Soler, N., Sanchez-Navarro, J., Fagoaga, C., Lopez, C. et al. (2013). Citrus tristeza virus p23: a unique protein mediating key virus-host interactions. Frontiers in Microbiology, 4, 98-98. 1664-302X http://hdl.handle.net/20.500.11939/5205 10.3389/fmicb.2013.00098 en openAccess Impreso |
| spellingShingle | Flores, Ricardo Ruiz-Ruiz, Susana Soler, Nuria Sánchez-Navarro, Jesús A. Fagoaga, Carmen Lopez, Carmelo Navarro, Luis Moreno, Pedro Pena, Leandro Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title | Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title_full | Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title_fullStr | Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title_full_unstemmed | Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title_short | Citrus tristeza virus p23: a unique protein mediating key virus-host interactions |
| title_sort | citrus tristeza virus p23 a unique protein mediating key virus host interactions |
| url | http://hdl.handle.net/20.500.11939/5205 |
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