Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes
Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection and identification. Also, detection techniques need to be rapid to keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is comp...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5200 |
| _version_ | 1855032241468473344 |
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| author | Ferriol, Inmaculada Rangel, Ezequiel A. Panno, Stefano Davino, Salvatore Han, Cheng-Gui Olmos, Antonio Rubio, Luis |
| author_browse | Davino, Salvatore Ferriol, Inmaculada Han, Cheng-Gui Olmos, Antonio Panno, Stefano Rangel, Ezequiel A. Rubio, Luis |
| author_facet | Ferriol, Inmaculada Rangel, Ezequiel A. Panno, Stefano Davino, Salvatore Han, Cheng-Gui Olmos, Antonio Rubio, Luis |
| author_sort | Ferriol, Inmaculada |
| collection | ReDivia |
| description | Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection and identification. Also, detection techniques need to be rapid to keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is composed of five viruses infecting many economically important crops worldwide. This research describes the development of a procedure based on flow-through hybridisation (FTH), which is faster than and as sensitive as conventional hybridisation for virus detection in tissue-prints from infected plants. Six digoxigenin-labelled RNA probes were synthesised with two levels of specificity: (a) five specific for each viral species within this genus, and (b) a genus-specific probe that hybridises with a nucleotide sequence signature only found in the 5-untranslated region of the genus Fabavirus, which is the first of this type reported for plant viruses. The new procedure developed is useful for rapid detection and discrimination of the five fabaviruses identified so far and opens the possibility of discovering new species of this genus. |
| format | article |
| id | ReDivia5200 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52002025-04-25T14:45:38Z Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes Ferriol, Inmaculada Rangel, Ezequiel A. Panno, Stefano Davino, Salvatore Han, Cheng-Gui Olmos, Antonio Rubio, Luis Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection and identification. Also, detection techniques need to be rapid to keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is composed of five viruses infecting many economically important crops worldwide. This research describes the development of a procedure based on flow-through hybridisation (FTH), which is faster than and as sensitive as conventional hybridisation for virus detection in tissue-prints from infected plants. Six digoxigenin-labelled RNA probes were synthesised with two levels of specificity: (a) five specific for each viral species within this genus, and (b) a genus-specific probe that hybridises with a nucleotide sequence signature only found in the 5-untranslated region of the genus Fabavirus, which is the first of this type reported for plant viruses. The new procedure developed is useful for rapid detection and discrimination of the five fabaviruses identified so far and opens the possibility of discovering new species of this genus. 2017-06-01T10:11:53Z 2017-06-01T10:11:53Z 2015 JUL 2015 article Ferriol, I., Rangel, E.A., Panno, S., Davino, S., Han, C. G., Olmos, A., Rubio, L. (2015). Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes. Annals of Applied Biology, 167(1), 26-35. 0003-4746 http://hdl.handle.net/20.500.11939/5200 10.1111/aab.12204 en openAccess Impreso |
| spellingShingle | Ferriol, Inmaculada Rangel, Ezequiel A. Panno, Stefano Davino, Salvatore Han, Cheng-Gui Olmos, Antonio Rubio, Luis Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title | Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title_full | Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title_fullStr | Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title_full_unstemmed | Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title_short | Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes |
| title_sort | rapid detection and discrimination of fabaviruses by flow through hybridisation with genus and species specific riboprobes |
| url | http://hdl.handle.net/20.500.11939/5200 |
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