Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers
The genus Fabavirus includes three species: Broad bean wilt virus 1 (BBWV-1), BBWV-2 and Lamium mild mosaic virus (LMMV), but a new candidate species, Gentian mosaic virus (GeMV), has been proposed. Analysis of the complete nucleotide sequences of fabaviruses was used to design a pair of conserved p...
| Main Authors: | , , , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5197 |
| _version_ | 1855032240924262400 |
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| author | Ferrer, Rosa M. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis |
| author_browse | Ferrer, Rosa M. Guerri, José Luis-Arteaga, Marisol Moreno, Pedro Rubio, Luis |
| author_facet | Ferrer, Rosa M. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis |
| author_sort | Ferrer, Rosa M. |
| collection | ReDivia |
| description | The genus Fabavirus includes three species: Broad bean wilt virus 1 (BBWV-1), BBWV-2 and Lamium mild mosaic virus (LMMV), but a new candidate species, Gentian mosaic virus (GeMV), has been proposed. Analysis of the complete nucleotide sequences of fabaviruses was used to design a pair of conserved primers for specific detection of members of this genus. These primers encompassed the 5'-terminal non-translatable region (NTR), whose size for BBWV-1, BBWV-2 and GeMV was different. RT-PCR, with this pair of primers, is a rapid and sensitive procedure for diagnosis of fabavirus infections, that also allows identification of distinct species involved in single or mixed infections, based on the size of the amplification products. Moreover, it might allow future discovery of potential new species of this genus. (c) 2007 Elsevier B.V. All rights reserved. |
| format | article |
| id | ReDivia5197 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51972025-04-25T14:45:37Z Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers Ferrer, Rosa M. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis The genus Fabavirus includes three species: Broad bean wilt virus 1 (BBWV-1), BBWV-2 and Lamium mild mosaic virus (LMMV), but a new candidate species, Gentian mosaic virus (GeMV), has been proposed. Analysis of the complete nucleotide sequences of fabaviruses was used to design a pair of conserved primers for specific detection of members of this genus. These primers encompassed the 5'-terminal non-translatable region (NTR), whose size for BBWV-1, BBWV-2 and GeMV was different. RT-PCR, with this pair of primers, is a rapid and sensitive procedure for diagnosis of fabavirus infections, that also allows identification of distinct species involved in single or mixed infections, based on the size of the amplification products. Moreover, it might allow future discovery of potential new species of this genus. (c) 2007 Elsevier B.V. All rights reserved. 2017-06-01T10:11:53Z 2017-06-01T10:11:53Z 2007 SEP 2007 article Ferrer, R. M., L.-Arteaga, M., Guerri, J., Moreno, P., Rubio, L. (2007). Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers. Journal of virological methods, 144(1-2), 156-160. 0166-0934 http://hdl.handle.net/20.500.11939/5197 10.1016/j.jviromet.2007.03.010 en openAccess Impreso |
| spellingShingle | Ferrer, Rosa M. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title | Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title_full | Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title_fullStr | Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title_full_unstemmed | Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title_short | Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers |
| title_sort | detection and identification of species of the genus fabavirus by rt pcr with a single pair of primers |
| url | http://hdl.handle.net/20.500.11939/5197 |
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