New molecular methods for identification of Broad bean wilt virus 1
Broad bean wilt virus 1 (BBWV-1) causes damages in economically important plant crops such as pepper, bean, spinach, etc. Fast, cheap and reliable diagnostic tools are crucial to limit or control the disease. In this work, tissue blot immunoassay (TBIA), dot-blot (DB) and tissue-print (TP)-hybridiza...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5195 |
| _version_ | 1855491883507122176 |
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| author | Ferrer, Rosa M. Escriu, F. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis |
| author_browse | Escriu, F. Ferrer, Rosa M. Guerri, José Luis-Arteaga, Marisol Moreno, Pedro Rubio, Luis |
| author_facet | Ferrer, Rosa M. Escriu, F. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis |
| author_sort | Ferrer, Rosa M. |
| collection | ReDivia |
| description | Broad bean wilt virus 1 (BBWV-1) causes damages in economically important plant crops such as pepper, bean, spinach, etc. Fast, cheap and reliable diagnostic tools are crucial to limit or control the disease. In this work, tissue blot immunoassay (TBIA), dot-blot (DB) and tissue-print (TP)-hybridization were developed for BBWV-1 diagnosis and evaluated for sensitivity, specificity and reliability in plants of several host species grown in the greenhouse or in the field, in comparison with ELISA and RT-PCR. RT-PCR followed by DB-hybridization provided the most sensitive and efficient diagnostic, but the virus was also detected in most samples by ELISA. Detection by TBIA or by TP-hybridization avoided sample processing, but they were less consistent and greatly depended on host species and tissue. DB-hybridization with probes corresponding to different genomic regions allowed universal detection of BBWV-1 and discrimination between genetically distant isolates. |
| format | Artículo |
| id | ReDivia5195 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51952025-04-25T14:45:37Z New molecular methods for identification of Broad bean wilt virus 1 Ferrer, Rosa M. Escriu, F. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis Broad bean wilt virus 1 (BBWV-1) causes damages in economically important plant crops such as pepper, bean, spinach, etc. Fast, cheap and reliable diagnostic tools are crucial to limit or control the disease. In this work, tissue blot immunoassay (TBIA), dot-blot (DB) and tissue-print (TP)-hybridization were developed for BBWV-1 diagnosis and evaluated for sensitivity, specificity and reliability in plants of several host species grown in the greenhouse or in the field, in comparison with ELISA and RT-PCR. RT-PCR followed by DB-hybridization provided the most sensitive and efficient diagnostic, but the virus was also detected in most samples by ELISA. Detection by TBIA or by TP-hybridization avoided sample processing, but they were less consistent and greatly depended on host species and tissue. DB-hybridization with probes corresponding to different genomic regions allowed universal detection of BBWV-1 and discrimination between genetically distant isolates. 2017-06-01T10:11:52Z 2017-06-01T10:11:52Z 2008 AUG 2008 article Ferrer, R. M., Escriu, F., Luis-Arteaga, M., Guerri, J., Moreno, P. et Rubio, L. (2008). New molecular methods for identification of Broad bean wilt virus 1. Molecular and cellular probes, 22(4), 223-227. 0890-8508 http://hdl.handle.net/20.500.11939/5195 10.1016/j.mcp.2008.02.004 en openAccess Impreso |
| spellingShingle | Ferrer, Rosa M. Escriu, F. Luis-Arteaga, Marisol Guerri, José Moreno, Pedro Rubio, Luis New molecular methods for identification of Broad bean wilt virus 1 |
| title | New molecular methods for identification of Broad bean wilt virus 1 |
| title_full | New molecular methods for identification of Broad bean wilt virus 1 |
| title_fullStr | New molecular methods for identification of Broad bean wilt virus 1 |
| title_full_unstemmed | New molecular methods for identification of Broad bean wilt virus 1 |
| title_short | New molecular methods for identification of Broad bean wilt virus 1 |
| title_sort | new molecular methods for identification of broad bean wilt virus 1 |
| url | http://hdl.handle.net/20.500.11939/5195 |
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