Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture
Carrizo citrange (Citrus sinensis x Poncirus trifoliata) is a citrus hybrid widely used as a rootstock, whose genetic manipulation to improve different growth characteristics is of high agronomic interest. In this work, transgenic Carrizo citrange plants have been produced overexpressing sense and a...
| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5178 |
| _version_ | 1855032238009221120 |
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| author | Fagoaga, Carmen Tadeo, Francisco R. Iglesias, Domingo J. Huerta, Laura Lliso, Ignacio Vidal, Ana M. Talón, Manuel Navarro, Luis García-Martínez, José Luis Pena, Leandro |
| author_browse | Fagoaga, Carmen García-Martínez, José Luis Huerta, Laura Iglesias, Domingo J. Lliso, Ignacio Navarro, Luis Pena, Leandro Tadeo, Francisco R. Talón, Manuel Vidal, Ana M. |
| author_facet | Fagoaga, Carmen Tadeo, Francisco R. Iglesias, Domingo J. Huerta, Laura Lliso, Ignacio Vidal, Ana M. Talón, Manuel Navarro, Luis García-Martínez, José Luis Pena, Leandro |
| author_sort | Fagoaga, Carmen |
| collection | ReDivia |
| description | Carrizo citrange (Citrus sinensis x Poncirus trifoliata) is a citrus hybrid widely used as a rootstock, whose genetic manipulation to improve different growth characteristics is of high agronomic interest. In this work, transgenic Carrizo citrange plants have been produced overexpressing sense and antisense CcGA20ox1 (a key enzyme of GA biosynthesis) under control of the 35S promoter to modify plant architecture. As expected, taller (sense) and shorter (antisense) phenotypes correlated with higher and lower levels, respectively, of active GA(1) in growing shoots. In contrast, other phenotypic characteristics seemed to be specific to citrus, or different from those described for similar transgenics in other species. For instance, thorns, typical organs of citrus at juvenile stages, were much longer in sense and shorter in antisense plants, and xylem tissue was reduced in leaf and internode of sense plants. Antisense plants presented a bushy phenotype, suggesting a possible effect of GAs on auxin biosynthesis and/or transport. The main foliole of sense plants was longer, although total leaf area was reduced. Leaf thickness was smaller in sense and larger in antisense plants due to changes in the spongy parenchyma. Internode cell length was not altered in transgenic plants, indicating that, in citrus, GAs regulate cell division rather than cell elongation. Interestingly, the phenotypes described were not apparent when transgenic plants were grafted on non-transgenic rootstock. This suggests that roots contribute to the GA economy of aerial parts in citrus and opens the possibility of using the antisense plants as dwarfing rootstocks. |
| format | article |
| id | ReDivia5178 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51782025-04-25T14:45:35Z Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture Fagoaga, Carmen Tadeo, Francisco R. Iglesias, Domingo J. Huerta, Laura Lliso, Ignacio Vidal, Ana M. Talón, Manuel Navarro, Luis García-Martínez, José Luis Pena, Leandro Carrizo citrange (Citrus sinensis x Poncirus trifoliata) is a citrus hybrid widely used as a rootstock, whose genetic manipulation to improve different growth characteristics is of high agronomic interest. In this work, transgenic Carrizo citrange plants have been produced overexpressing sense and antisense CcGA20ox1 (a key enzyme of GA biosynthesis) under control of the 35S promoter to modify plant architecture. As expected, taller (sense) and shorter (antisense) phenotypes correlated with higher and lower levels, respectively, of active GA(1) in growing shoots. In contrast, other phenotypic characteristics seemed to be specific to citrus, or different from those described for similar transgenics in other species. For instance, thorns, typical organs of citrus at juvenile stages, were much longer in sense and shorter in antisense plants, and xylem tissue was reduced in leaf and internode of sense plants. Antisense plants presented a bushy phenotype, suggesting a possible effect of GAs on auxin biosynthesis and/or transport. The main foliole of sense plants was longer, although total leaf area was reduced. Leaf thickness was smaller in sense and larger in antisense plants due to changes in the spongy parenchyma. Internode cell length was not altered in transgenic plants, indicating that, in citrus, GAs regulate cell division rather than cell elongation. Interestingly, the phenotypes described were not apparent when transgenic plants were grafted on non-transgenic rootstock. This suggests that roots contribute to the GA economy of aerial parts in citrus and opens the possibility of using the antisense plants as dwarfing rootstocks. 2017-06-01T10:11:51Z 2017-06-01T10:11:51Z 2007 2007 article Fagoaga, C., Tadeo, F.R., Iglesias, D.J., Huerta, L., Lliso, I., Vidal, Ana M., Talon, M., Navarro, L., Garcia-Martinez, J.L., Pena, L. (2007). Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture. Journal of experimental botany, 58(6), 1407-1420. 0022-0957 http://hdl.handle.net/20.500.11939/5178 10.1093/jxb/erm004 en openAccess Impreso |
| spellingShingle | Fagoaga, Carmen Tadeo, Francisco R. Iglesias, Domingo J. Huerta, Laura Lliso, Ignacio Vidal, Ana M. Talón, Manuel Navarro, Luis García-Martínez, José Luis Pena, Leandro Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title | Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title_full | Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title_fullStr | Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title_full_unstemmed | Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title_short | Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture |
| title_sort | engineering of gibberellin levels in citrus by sense and antisense overexpression of a ga 20 oxidase gene modifies plant architecture |
| url | http://hdl.handle.net/20.500.11939/5178 |
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