Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5
Phytophthora citrophthora is the most widely spread oomycete plant pathogen over all the citrus growing areas and represents one of the major causes of crop losses. Constitutive over-expression of genes encoding proteins involved in plant defence mechanisms to disease is one of the strategies propos...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5176 |
| _version_ | 1855032237682065408 |
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| author | Fagoaga, Carmen Rodrigo, I. Conejero, Vicente Hinarejos, Concha Tuset, Juan J. Arnau, J. Pina, José A. Navarro, Luis Pena, Leandro |
| author_browse | Arnau, J. Conejero, Vicente Fagoaga, Carmen Hinarejos, Concha Navarro, Luis Pena, Leandro Pina, José A. Rodrigo, I. Tuset, Juan J. |
| author_facet | Fagoaga, Carmen Rodrigo, I. Conejero, Vicente Hinarejos, Concha Tuset, Juan J. Arnau, J. Pina, José A. Navarro, Luis Pena, Leandro |
| author_sort | Fagoaga, Carmen |
| collection | ReDivia |
| description | Phytophthora citrophthora is the most widely spread oomycete plant pathogen over all the citrus growing areas and represents one of the major causes of crop losses. Constitutive over-expression of genes encoding proteins involved in plant defence mechanisms to disease is one of the strategies proposed to increase plant tolerance to oomycete and fungal pathogens. P23 (PR-5), a 23-kDa pathogenesis-related protein similar to osmotins, is induced in tomato (Lycopersicon esculentum Mill. cv. Rutgers) plants when they are infected with citrus exocortis viroid, and its antifungal activity has been demonstrated in in vitro assays. We have successfully produced transgenic orange (Citrus sinensis L. Obs. cv. Pineapple) plants bearing a chimeric gene construct consisting of the cauliflower mosaic virus 35S promoter and the coding region of the tomato pathogenesis-related PR-5. Nine regenerated transgenic lines constitutively expressed the PR protein. They were challenged with Phytophthora citrophthora using a detached bark assay. A significant reduction in lesion development was consistently observed in one transgenic line in comparison to the control plants. This same line achieved plant survival rates higher than control plants when transgenic trees were inoculated with oomycete cultures. These results provide evidence for the in vivo activity of the tomato PR-5 protein against Phytophthora citrophthora, and suggest that this may be employed as a strategy aimed at engineering Phytophthora disease resistance in citrus. |
| format | article |
| id | ReDivia5176 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51762025-04-25T14:45:35Z Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 Fagoaga, Carmen Rodrigo, I. Conejero, Vicente Hinarejos, Concha Tuset, Juan J. Arnau, J. Pina, José A. Navarro, Luis Pena, Leandro Phytophthora citrophthora is the most widely spread oomycete plant pathogen over all the citrus growing areas and represents one of the major causes of crop losses. Constitutive over-expression of genes encoding proteins involved in plant defence mechanisms to disease is one of the strategies proposed to increase plant tolerance to oomycete and fungal pathogens. P23 (PR-5), a 23-kDa pathogenesis-related protein similar to osmotins, is induced in tomato (Lycopersicon esculentum Mill. cv. Rutgers) plants when they are infected with citrus exocortis viroid, and its antifungal activity has been demonstrated in in vitro assays. We have successfully produced transgenic orange (Citrus sinensis L. Obs. cv. Pineapple) plants bearing a chimeric gene construct consisting of the cauliflower mosaic virus 35S promoter and the coding region of the tomato pathogenesis-related PR-5. Nine regenerated transgenic lines constitutively expressed the PR protein. They were challenged with Phytophthora citrophthora using a detached bark assay. A significant reduction in lesion development was consistently observed in one transgenic line in comparison to the control plants. This same line achieved plant survival rates higher than control plants when transgenic trees were inoculated with oomycete cultures. These results provide evidence for the in vivo activity of the tomato PR-5 protein against Phytophthora citrophthora, and suggest that this may be employed as a strategy aimed at engineering Phytophthora disease resistance in citrus. 2017-06-01T10:11:51Z 2017-06-01T10:11:51Z 2001 article Fagoaga, C., Rodrigo, I., Conejero, V., Hinarejos, C., Tuset, J. J., Arnau, J., Pina, JA, Navarro, L., Pena, L. (2001). Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5. Molecular Breeding, 7(2), 175-185. 1380-3743 http://hdl.handle.net/20.500.11939/5176 10.1023/A:1011358005054 en openAccess Impreso |
| spellingShingle | Fagoaga, Carmen Rodrigo, I. Conejero, Vicente Hinarejos, Concha Tuset, Juan J. Arnau, J. Pina, José A. Navarro, Luis Pena, Leandro Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title | Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title_full | Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title_fullStr | Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title_full_unstemmed | Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title_short | Increased tolerance to Phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein PR-5 |
| title_sort | increased tolerance to phytophthora citrophthora in transgenic orange plants constitutively expressing a tomato pathogenesis related protein pr 5 |
| url | http://hdl.handle.net/20.500.11939/5176 |
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