Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime
Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5171 |
| _version_ | 1855491879828717568 |
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| author | Fagoaga, Carmen Lopez, C. Hermoso-De-Mendoza, Alfonso Moreno, Pedro Navarro, Luis Flores, R. Pena, Leandro |
| author_browse | Fagoaga, Carmen Flores, R. Hermoso-De-Mendoza, Alfonso Lopez, C. Moreno, Pedro Navarro, Luis Pena, Leandro |
| author_facet | Fagoaga, Carmen Lopez, C. Hermoso-De-Mendoza, Alfonso Moreno, Pedro Navarro, Luis Flores, R. Pena, Leandro |
| author_sort | Fagoaga, Carmen |
| collection | ReDivia |
| description | Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific small interfering RNAs (siRNAs). When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. Other propagations were moderately resistant because they became infected later and showed attenuated symptoms compared to controls. The susceptible propagations, in addition to symptom expression and elevated virus titer, accumulated p23-specific siRNAs at levels significantly higher than immune or non-inoculated propagations, and showed transgene demethylation. This variable response among clonal transformants indicates that factors other than the genetic background of the transgenic plants play a key role in PTGS-mediated resistance. |
| format | Artículo |
| id | ReDivia5171 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51712025-04-25T14:45:34Z Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime Fagoaga, Carmen Lopez, C. Hermoso-De-Mendoza, Alfonso Moreno, Pedro Navarro, Luis Flores, R. Pena, Leandro Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific small interfering RNAs (siRNAs). When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. Other propagations were moderately resistant because they became infected later and showed attenuated symptoms compared to controls. The susceptible propagations, in addition to symptom expression and elevated virus titer, accumulated p23-specific siRNAs at levels significantly higher than immune or non-inoculated propagations, and showed transgene demethylation. This variable response among clonal transformants indicates that factors other than the genetic background of the transgenic plants play a key role in PTGS-mediated resistance. 2017-06-01T10:11:50Z 2017-06-01T10:11:50Z 2006 JAN 2006 article Fagoaga, C., Lopez, C., Hermoso-de-Mendoza, A., Moreno, P., Navarro, L., Flores, R., Pena, L. (2006). Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime. Plant Molecular Biology, 60(2), 153-165. 0167-4412 http://hdl.handle.net/20.500.11939/5171 10.1007/s11103-005-3129-7 en openAccess Impreso |
| spellingShingle | Fagoaga, Carmen Lopez, C. Hermoso-De-Mendoza, Alfonso Moreno, Pedro Navarro, Luis Flores, R. Pena, Leandro Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title | Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title_full | Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title_fullStr | Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title_full_unstemmed | Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title_short | Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime |
| title_sort | post transcriptional gene silencing of the p23 silencing suppressor of citrus tristeza virus confers resistance to the virus in transgenic mexican lime |
| url | http://hdl.handle.net/20.500.11939/5171 |
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