Generation and characterisation of functional recombinant antibody fragments against RNA replicase NIb from plum pox virus

A monoclonal antibody (mAb 2A) able to react against the RNA replicase Nib from plum pox virus (PPV) was obtained and used for generating a specific scFv fragment. The VH and VL coding sequences were cloned and expressed as a fusion scFv protein to alkaline phosphatase. This fusion protein was able...

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Bibliographic Details
Main Authors: Esteban, Olga, Garcia, Juan Antonio, Gorris, María T., Dominguez, E., Cambra, Mariano
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5164

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