Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit
We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harbor...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5161 |
| _version_ | 1855032235517804544 |
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| author | Endo, Tomoko Shimada, Takehiko Fujii, Hiroshi Nishikawa, Fumie Sugiyama, Aiko Nakano, Michiharu Shimizu, Tokurou Kobayashi, Yasushi Araki, Takashi Pena, Leandro Omura, Mitsuo |
| author_browse | Araki, Takashi Endo, Tomoko Fujii, Hiroshi Kobayashi, Yasushi Nakano, Michiharu Nishikawa, Fumie Omura, Mitsuo Pena, Leandro Shimada, Takehiko Shimizu, Tokurou Sugiyama, Aiko |
| author_facet | Endo, Tomoko Shimada, Takehiko Fujii, Hiroshi Nishikawa, Fumie Sugiyama, Aiko Nakano, Michiharu Shimizu, Tokurou Kobayashi, Yasushi Araki, Takashi Pena, Leandro Omura, Mitsuo |
| author_sort | Endo, Tomoko |
| collection | ReDivia |
| description | We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate orange (Poncirus trifoliata L. Raf). In order to reduce the limonene level, a gene encoding limonene synthase (CitMTSE1) from satsuma mandarin (Citrus unshiu Marc.) was introduced into trifolliate orange in an anti-sense orientation with the CiFT co-expression vector. Transgenic plants flowered extremely early, and began to produce normal fruit within 2 years of Agrobacterium infection. Transcripts of transgenes were accumulated in reproductive tissues of transgenic plants, and endogenous transcripts for limonene synthase were reduced. The ratio of limonene to other monoterpenes was significantly decreased in flowers and fruit of transgenic plants. These results clearly indicated that the CiFT co-expression system is a useful tool for the functional analysis of genes in citrus flowers and fruit. Such analyses are important for several applications, including the metabolic engineering of aroma components in citrus. |
| format | article |
| id | ReDivia5161 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51612025-04-25T14:45:32Z Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit Endo, Tomoko Shimada, Takehiko Fujii, Hiroshi Nishikawa, Fumie Sugiyama, Aiko Nakano, Michiharu Shimizu, Tokurou Kobayashi, Yasushi Araki, Takashi Pena, Leandro Omura, Mitsuo We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate orange (Poncirus trifoliata L. Raf). In order to reduce the limonene level, a gene encoding limonene synthase (CitMTSE1) from satsuma mandarin (Citrus unshiu Marc.) was introduced into trifolliate orange in an anti-sense orientation with the CiFT co-expression vector. Transgenic plants flowered extremely early, and began to produce normal fruit within 2 years of Agrobacterium infection. Transcripts of transgenes were accumulated in reproductive tissues of transgenic plants, and endogenous transcripts for limonene synthase were reduced. The ratio of limonene to other monoterpenes was significantly decreased in flowers and fruit of transgenic plants. These results clearly indicated that the CiFT co-expression system is a useful tool for the functional analysis of genes in citrus flowers and fruit. Such analyses are important for several applications, including the metabolic engineering of aroma components in citrus. 2017-06-01T10:11:49Z 2017-06-01T10:11:49Z 2009 JAN 2009 article publishedVersion Endo, Tomoko, Shimada, Takehiko, Fujii, Hiroshi, Nishikawa, Fumie, Sugiyama, Aiko, Nakano, Michiharu, Shimizu, Tokurou, Kobayashi, Yasushi, Araki, Takashi, Pena, L., Omura, Mitsuo (2009). Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit. Journal of the Japanese Society for Horticultural Science, 78(1), 74-83. 1882-3351 http://hdl.handle.net/20.500.11939/5161 http://hdl.handle.net/20.500.11939/5161 10.2503/jjshs1.78.74 en openAccess Impreso |
| spellingShingle | Endo, Tomoko Shimada, Takehiko Fujii, Hiroshi Nishikawa, Fumie Sugiyama, Aiko Nakano, Michiharu Shimizu, Tokurou Kobayashi, Yasushi Araki, Takashi Pena, Leandro Omura, Mitsuo Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title | Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title_full | Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title_fullStr | Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title_full_unstemmed | Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title_short | Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit |
| title_sort | development of a cift co expression system for functional analysis of genes in citrus flowers and fruit |
| url | http://hdl.handle.net/20.500.11939/5161 http://hdl.handle.net/20.500.11939/5161 |
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