Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation
Both incubation temperature and photosynthetic radiation affected morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis) cultured in vitro. Bud culture from nodal stem segments, regeneration of shoots and buds from internode stem segments and induction of primary callus...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5152 |
| _version_ | 1855032234071818240 |
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| author | Durán-Vila, Núria Gogorcena, Y. Ortega, Violeta Ortiz Marcide, Jesús M. Navarro, Luis |
| author_browse | Durán-Vila, Núria Gogorcena, Y. Navarro, Luis Ortega, Violeta Ortiz Marcide, Jesús M. |
| author_facet | Durán-Vila, Núria Gogorcena, Y. Ortega, Violeta Ortiz Marcide, Jesús M. Navarro, Luis |
| author_sort | Durán-Vila, Núria |
| collection | ReDivia |
| description | Both incubation temperature and photosynthetic radiation affected morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis) cultured in vitro. Bud culture from nodal stem segments, regeneration of shoots and buds from internode stem segments and induction of primary callus were near optimal at incubation temperatures between 21-30-degrees-C. The optimal temperature for root formation was 27-degrees-C with temperatures above and below being clearly deleterious. Incubation in the dark or under low photosynthetic photon flux density (PPFD) was beneficial for callus induction and growth and also favored the production of rooted plantlets from bud cultures. Incubation in the dark improved considerably the regeneration of shoots and buds from internode segments and the recovery of whole plants. No off-types, as determined by protein and isoenzyme analysis, were observed among plantlets recovered from bud cultures or from regeneration of shoots from internode stem segments. |
| format | article |
| id | ReDivia5152 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51522025-04-25T14:45:31Z Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation Durán-Vila, Núria Gogorcena, Y. Ortega, Violeta Ortiz Marcide, Jesús M. Navarro, Luis Both incubation temperature and photosynthetic radiation affected morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis) cultured in vitro. Bud culture from nodal stem segments, regeneration of shoots and buds from internode stem segments and induction of primary callus were near optimal at incubation temperatures between 21-30-degrees-C. The optimal temperature for root formation was 27-degrees-C with temperatures above and below being clearly deleterious. Incubation in the dark or under low photosynthetic photon flux density (PPFD) was beneficial for callus induction and growth and also favored the production of rooted plantlets from bud cultures. Incubation in the dark improved considerably the regeneration of shoots and buds from internode segments and the recovery of whole plants. No off-types, as determined by protein and isoenzyme analysis, were observed among plantlets recovered from bud cultures or from regeneration of shoots from internode stem segments. 2017-06-01T10:11:48Z 2017-06-01T10:11:48Z 1992 APR article Durán-Vila, N., Gogorcena, Y., Ortega, V., Ortiz, J. M. & Navarro, L. (1992). Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L), Osb), - Effect of Temperature and Photosynthetic Radiation. Plant Cell Tissue and Organ Culture, 29(1), 11-18. 0167-6857 http://hdl.handle.net/20.500.11939/5152 10.1007/BF00036140 en openAccess Impreso |
| spellingShingle | Durán-Vila, Núria Gogorcena, Y. Ortega, Violeta Ortiz Marcide, Jesús M. Navarro, Luis Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title | Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title_full | Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title_fullStr | Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title_full_unstemmed | Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title_short | Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation |
| title_sort | morphogenesis and tissue culture of sweet orange citrus sinensis l osb effect of temperature and photosynthetic radiation |
| url | http://hdl.handle.net/20.500.11939/5152 |
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