Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments

Loquat (Eriobotrya japonica (Thunb.) Lindl.) is a minor Rosaceae fruit of growing interest as an alternative to the main fruit crops. In this context, the selection of new cultivars to satisfy the market demand will request the suitable characterization of the available germplasm. In this work, gene...

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Main Authors: Gisbert, Ana D., Romero, Carlos, Martínez-Calvo, José, Leida, Carmen, Llácer, Gerardo, Badenes, María L.
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/5122
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author Gisbert, Ana D.
Romero, Carlos
Martínez-Calvo, José
Leida, Carmen
Llácer, Gerardo
Badenes, María L.
author_browse Badenes, María L.
Gisbert, Ana D.
Leida, Carmen
Llácer, Gerardo
Martínez-Calvo, José
Romero, Carlos
author_facet Gisbert, Ana D.
Romero, Carlos
Martínez-Calvo, José
Leida, Carmen
Llácer, Gerardo
Badenes, María L.
author_sort Gisbert, Ana D.
collection ReDivia
description Loquat (Eriobotrya japonica (Thunb.) Lindl.) is a minor Rosaceae fruit of growing interest as an alternative to the main fruit crops. In this context, the selection of new cultivars to satisfy the market demand will request the suitable characterization of the available germplasm. In this work, genetic relationships among 83 loquat accessions from different countries belonging to the European loquat germplasm collection, held at the Instituto Valenciano de Investigaciones Agrarias (IVIA) in Moncada (Spain) were evaluated using microsatellites and S-allele fragments. A total of nine single sequence repeats (SSRs) from Malus and Eriobotrya genera revealed 53 informative alleles and the S-RNases consensus primers detected 11 self-incompatibility putative alleles. The combined data allow to distinguish unambiguously 80 out of the 83 accessions studied. Unweighted pair-group method (UPGMA) cluster and principal coordinates analysis (PCoA), based on Dice’s genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. Discrepancies and similarities of the results obtained with other variability analysis, based on pomological traits or molecular markers, on the same loquat collection are discussed.
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spelling ReDivia51222025-04-25T14:45:26Z Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments Gisbert, Ana D. Romero, Carlos Martínez-Calvo, José Leida, Carmen Llácer, Gerardo Badenes, María L. Loquat (Eriobotrya japonica (Thunb.) Lindl.) is a minor Rosaceae fruit of growing interest as an alternative to the main fruit crops. In this context, the selection of new cultivars to satisfy the market demand will request the suitable characterization of the available germplasm. In this work, genetic relationships among 83 loquat accessions from different countries belonging to the European loquat germplasm collection, held at the Instituto Valenciano de Investigaciones Agrarias (IVIA) in Moncada (Spain) were evaluated using microsatellites and S-allele fragments. A total of nine single sequence repeats (SSRs) from Malus and Eriobotrya genera revealed 53 informative alleles and the S-RNases consensus primers detected 11 self-incompatibility putative alleles. The combined data allow to distinguish unambiguously 80 out of the 83 accessions studied. Unweighted pair-group method (UPGMA) cluster and principal coordinates analysis (PCoA), based on Dice’s genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. Discrepancies and similarities of the results obtained with other variability analysis, based on pomological traits or molecular markers, on the same loquat collection are discussed. 2017-06-01T10:11:44Z 2017-06-01T10:11:44Z 2009 JUL 2009 article Gisbert, D. A., Romero, C., Martinez-Calvo, J., Leida, C., Llacer, G. & Badenes, M. L. (2009). Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb), Lindl), germplasm collection by SSRs and S-allele fragments. Euphytica, 168(1), 121-134. 0014-2336 http://hdl.handle.net/20.500.11939/5122 10.1007/s10681-009-9901-z en openAccess Impreso
spellingShingle Gisbert, Ana D.
Romero, Carlos
Martínez-Calvo, José
Leida, Carmen
Llácer, Gerardo
Badenes, María L.
Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title_full Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title_fullStr Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title_full_unstemmed Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title_short Genetic diversity evaluation of a loquat (Eriobotrya japonica (Thunb) Lindl) germplasm collection by SSRs and S-allele fragments
title_sort genetic diversity evaluation of a loquat eriobotrya japonica thunb lindl germplasm collection by ssrs and s allele fragments
url http://hdl.handle.net/20.500.11939/5122
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