Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain
Tomato spotted wilt virus (TSWV) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method so far for TSWV disease control, but only two genes have been found to confer resistance ag...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5112 |
| _version_ | 1855032227542335488 |
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| author | Debreczeni, Diana E. Lopez, Carmelo Aramburu, Jose Daros, José A. Soler, Salvador Galipienso, Luis Falk, Bryce W. Rubio, Luis |
| author_browse | Aramburu, Jose Daros, José A. Debreczeni, Diana E. Falk, Bryce W. Galipienso, Luis Lopez, Carmelo Rubio, Luis Soler, Salvador |
| author_facet | Debreczeni, Diana E. Lopez, Carmelo Aramburu, Jose Daros, José A. Soler, Salvador Galipienso, Luis Falk, Bryce W. Rubio, Luis |
| author_sort | Debreczeni, Diana E. |
| collection | ReDivia |
| description | Tomato spotted wilt virus (TSWV) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method so far for TSWV disease control, but only two genes have been found to confer resistance against a wide spectrum of TSWV isolates: Sw-5 in tomato and Tsw in pepper. However, TSWV resistance-breaking isolates have emerged in different countries a few years after using resistant cultivars. In this paper, we report the first complete nucleotide sequences of three Spanish TSWV isolates with different biotypes according to their abilities to overcome resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 resistance breaking, SBR) and PVR (Tsw resistance-breaking, TBR). The genome of these TSWV isolates consisted of three segments: L (8913-8914 nt), M (4752-4825 nt) and (S 2924-2961 nt). Variations in nucleotide sequences and genomic RNA lengths among the different virus biotypes are reported here. Phylogenetic analysis of the five TSWV open reading frames showed evidence of reassortment between genomic segments of LL-N.05 and Pujol1TL3, which was supported by analysis with different recombination-detecting algorithms. |
| format | article |
| id | ReDivia5112 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia51122025-04-25T14:45:24Z Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain Debreczeni, Diana E. Lopez, Carmelo Aramburu, Jose Daros, José A. Soler, Salvador Galipienso, Luis Falk, Bryce W. Rubio, Luis Tomato spotted wilt virus (TSWV) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method so far for TSWV disease control, but only two genes have been found to confer resistance against a wide spectrum of TSWV isolates: Sw-5 in tomato and Tsw in pepper. However, TSWV resistance-breaking isolates have emerged in different countries a few years after using resistant cultivars. In this paper, we report the first complete nucleotide sequences of three Spanish TSWV isolates with different biotypes according to their abilities to overcome resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 resistance breaking, SBR) and PVR (Tsw resistance-breaking, TBR). The genome of these TSWV isolates consisted of three segments: L (8913-8914 nt), M (4752-4825 nt) and (S 2924-2961 nt). Variations in nucleotide sequences and genomic RNA lengths among the different virus biotypes are reported here. Phylogenetic analysis of the five TSWV open reading frames showed evidence of reassortment between genomic segments of LL-N.05 and Pujol1TL3, which was supported by analysis with different recombination-detecting algorithms. 2017-06-01T10:11:44Z 2017-06-01T10:11:44Z 2015 AUG 2015 article acceptedVersion Debreczeni, Diana E., Lopez, Carmelo, Aramburu, J., A. Daros, J., Soler, Salvador, Galipienso, L., Falk, Bryce W., Rubio, L. (2015). Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking), from Spain. Archives of Virology, 160(8), 2117-2123. 0304-8608 http://hdl.handle.net/20.500.11939/5112 10.1007/s00705-015-2453-8 10.1007/s00705-016-3177-0 en openAccess Impreso |
| spellingShingle | Debreczeni, Diana E. Lopez, Carmelo Aramburu, Jose Daros, José A. Soler, Salvador Galipienso, Luis Falk, Bryce W. Rubio, Luis Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title | Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title_full | Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title_fullStr | Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title_full_unstemmed | Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title_short | Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain |
| title_sort | complete sequence of three different biotypes of tomato spotted wilt virus wild type tomato sw 5 resistance breaking and pepper tsw resistance breaking from spain |
| url | http://hdl.handle.net/20.500.11939/5112 |
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