Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry
The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5082 |
| _version_ | 1855032222982078464 |
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| author | Dambier, Dominique Benyahia, Hamid Pensabene-Bellavia, Giovanni Kacar, Yildiz Aka Froelicher, Yann Belfalah, Zina Lhou, Beniken Handaji, Najat Printz, Bruno Morillon, Raphael Yesiloglu, Turgut Navarro, Luis Ollitrault, Patrick |
| author_browse | Belfalah, Zina Benyahia, Hamid Dambier, Dominique Froelicher, Yann Handaji, Najat Kacar, Yildiz Aka Lhou, Beniken Morillon, Raphael Navarro, Luis Ollitrault, Patrick Pensabene-Bellavia, Giovanni Printz, Bruno Yesiloglu, Turgut |
| author_facet | Dambier, Dominique Benyahia, Hamid Pensabene-Bellavia, Giovanni Kacar, Yildiz Aka Froelicher, Yann Belfalah, Zina Lhou, Beniken Handaji, Najat Printz, Bruno Morillon, Raphael Yesiloglu, Turgut Navarro, Luis Ollitrault, Patrick |
| author_sort | Dambier, Dominique |
| collection | ReDivia |
| description | The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for alternative CTV-resistant rootstocks that also withstand the other constraints is now considered an urgent priority for a sustainable citrus industry in the area. Complementary progenitors can be found in citrus germplasm to combine the desired traits, particularly between Poncirus and Citrus genera. The production of somatic hybrids allows cumulating all dominant traits irrespective of their heterozygosity level, and would appear to be an effective way to solve the rootstock challenge facing the Mediterranean citrus industry. This paper presents the results obtained during a regional collaborative effort between five countries, to develop new rootstocks by somatic hybridization. New embryogenic callus lines to be used for somatic hybridization have been created. Protoplast fusions have been performed at CIRAD and IVIA laboratories, focusing on intergeneric combinations. Analysis of ploidy level by flow cytometry and molecular markers confirmed the acquisition of new interesting tetraploid somatic hybrids for six combinations. Diploid cybrids with intergeneric (Citrus x Poncirus) nucleus and C. reticulata or C. aurantifolia mitochondria were also identified for four combinations. The agronomical performance of a pre-existing somatic hybrid between Poncirus trifoliata and Citrus reticulata was validated in calcareous soils in Morocco. Somatic hybridization is now integrated into the breeding programs of the five Mediterranean countries. |
| format | article |
| id | ReDivia5082 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50822025-04-25T14:45:20Z Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry Dambier, Dominique Benyahia, Hamid Pensabene-Bellavia, Giovanni Kacar, Yildiz Aka Froelicher, Yann Belfalah, Zina Lhou, Beniken Handaji, Najat Printz, Bruno Morillon, Raphael Yesiloglu, Turgut Navarro, Luis Ollitrault, Patrick The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for alternative CTV-resistant rootstocks that also withstand the other constraints is now considered an urgent priority for a sustainable citrus industry in the area. Complementary progenitors can be found in citrus germplasm to combine the desired traits, particularly between Poncirus and Citrus genera. The production of somatic hybrids allows cumulating all dominant traits irrespective of their heterozygosity level, and would appear to be an effective way to solve the rootstock challenge facing the Mediterranean citrus industry. This paper presents the results obtained during a regional collaborative effort between five countries, to develop new rootstocks by somatic hybridization. New embryogenic callus lines to be used for somatic hybridization have been created. Protoplast fusions have been performed at CIRAD and IVIA laboratories, focusing on intergeneric combinations. Analysis of ploidy level by flow cytometry and molecular markers confirmed the acquisition of new interesting tetraploid somatic hybrids for six combinations. Diploid cybrids with intergeneric (Citrus x Poncirus) nucleus and C. reticulata or C. aurantifolia mitochondria were also identified for four combinations. The agronomical performance of a pre-existing somatic hybrid between Poncirus trifoliata and Citrus reticulata was validated in calcareous soils in Morocco. Somatic hybridization is now integrated into the breeding programs of the five Mediterranean countries. 2017-06-01T10:11:41Z 2017-06-01T10:11:41Z 2011 MAY article Dambier, Dominique, Benyahia, Hamid, Pensabene-Bellavia, Giovanni, Kacar, Yildiz Aka, Froelicher, Yann, Belfalah, Zina, Lhou, Beniken, Handaji, Najat, Printz, Bruno, Morillon, R., Yesiloglu, Turgut, Navarro, L., Ollitrault, P. (2011). Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry. Plant Cell Reports, 30(5), 883-900. 0721-7714 http://hdl.handle.net/20.500.11939/5082 10.1007/s00299-010-1000-z en openAccess Impreso |
| spellingShingle | Dambier, Dominique Benyahia, Hamid Pensabene-Bellavia, Giovanni Kacar, Yildiz Aka Froelicher, Yann Belfalah, Zina Lhou, Beniken Handaji, Najat Printz, Bruno Morillon, Raphael Yesiloglu, Turgut Navarro, Luis Ollitrault, Patrick Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title_full | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title_fullStr | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title_full_unstemmed | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title_short | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry |
| title_sort | somatic hybridization for citrus rootstock breeding an effective tool to solve some important issues of the mediterranean citrus industry |
| url | http://hdl.handle.net/20.500.11939/5082 |
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