Assignment of SNP Allelic Configuration in Polyploids Using Competitive Allele-Specific PCR: Application to Citrus Triploid Progenies
Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole genome coverage of SNP markers, it is important to implement a versatile SNP genot...
| Autores principales: | , , , |
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| Otros Autores: | |
| Formato: | conferenceObject |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5072 https://doi.org/10.17660/ActaHortic.2015.1065.53 |
| Sumario: | Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole genome coverage of SNP markers, it is important to implement a versatile SNP genotyping method to efficiently assign allelic configuration in polyploids. This work demonstrates the usefulness of the KASPar method, based on competitive allele-specific PCR, for the assignment of SNP allelic configuration in an efficient, accurate, simple, and cost effective way and it has been successfully applied in genotyping citrus triploids. |
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