Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus
Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5069 |
| _version_ | 1855032220658434048 |
|---|---|
| author | Cuenca, José Aleza, Pablo Juárez, José García-Lor, Andrés Froelicher, Yann Navarro, Luis Ollitrault, Patrick |
| author_browse | Aleza, Pablo Cuenca, José Froelicher, Yann García-Lor, Andrés Juárez, José Navarro, Luis Ollitrault, Patrick |
| author_facet | Cuenca, José Aleza, Pablo Juárez, José García-Lor, Andrés Froelicher, Yann Navarro, Luis Ollitrault, Patrick |
| author_sort | Cuenca, José |
| collection | ReDivia |
| description | Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins. |
| format | article |
| id | ReDivia5069 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50692025-04-25T14:45:17Z Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus Cuenca, José Aleza, Pablo Juárez, José García-Lor, Andrés Froelicher, Yann Navarro, Luis Ollitrault, Patrick Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins. 2017-06-01T10:11:39Z 2017-06-01T10:11:39Z 2015 APR 20 article publishedVersion Cuenca, J., Aleza, P., Juarez, J., García-Lor, A., Froelicher, Y., Navarro, L., & Ollitrault, P. (2015). Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus. Scientific reports, 5, 9897. 2045-2322 http://hdl.handle.net/20.500.11939/5069 10.1038/srep09897 en openAccess Impreso |
| spellingShingle | Cuenca, José Aleza, Pablo Juárez, José García-Lor, Andrés Froelicher, Yann Navarro, Luis Ollitrault, Patrick Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title | Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title_full | Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title_fullStr | Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title_full_unstemmed | Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title_short | Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus |
| title_sort | maximum likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci application in citrus |
| url | http://hdl.handle.net/20.500.11939/5069 |
| work_keys_str_mv | AT cuencajose maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT alezapablo maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT juarezjose maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT garcialorandres maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT froelicheryann maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT navarroluis maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus AT ollitraultpatrick maximumlikelihoodmethodidentifiesmeioticrestitutionmechanismfromheterozygositytransmissionofcentromericlociapplicationincitrus |