An internal control for the diagnosis of crown gall by PCR
The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown call diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the at...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5058 |
| _version_ | 1855491860034748416 |
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| author | Cubero, Jaime van der Wolf, J. M. van Beckhoven, J. López, María M. |
| author_browse | Cubero, Jaime López, María M. van Beckhoven, J. van der Wolf, J. M. |
| author_facet | Cubero, Jaime van der Wolf, J. M. van Beckhoven, J. López, María M. |
| author_sort | Cubero, Jaime |
| collection | ReDivia |
| description | The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown call diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified. |
| format | Artículo |
| id | ReDivia5058 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50582025-04-25T14:45:16Z An internal control for the diagnosis of crown gall by PCR Cubero, Jaime van der Wolf, J. M. van Beckhoven, J. López, María M. The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown call diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified. 2017-06-01T10:11:37Z 2017-06-01T10:11:37Z 2002 NOV 2002 article Cubero, J., van der Wolf, J., van Beckhoven, J. & Lopez, M. M. (2002). An internal control for the diagnosis of crown gall by PCR. Journal of microbiological methods, 51(3), 387-392. 0167-7012 http://hdl.handle.net/20.500.11939/5058 10.1016/S0167-7012(02)00153-7 en openAccess Impreso |
| spellingShingle | Cubero, Jaime van der Wolf, J. M. van Beckhoven, J. López, María M. An internal control for the diagnosis of crown gall by PCR |
| title | An internal control for the diagnosis of crown gall by PCR |
| title_full | An internal control for the diagnosis of crown gall by PCR |
| title_fullStr | An internal control for the diagnosis of crown gall by PCR |
| title_full_unstemmed | An internal control for the diagnosis of crown gall by PCR |
| title_short | An internal control for the diagnosis of crown gall by PCR |
| title_sort | internal control for the diagnosis of crown gall by pcr |
| url | http://hdl.handle.net/20.500.11939/5058 |
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